Stefano Vitória C, Loiola Saulo Hn, Dos Santos Bianca M, Soares Felipe A, Rosa Stefany L, Suzuki Celso Tn, Bresciani Katia Ds, Sabadini Edvaldo, Falcão Alexandre X, Gomes Jancarlo F
Laboratory of Image Data Science, Institute of Computing, University of Campinas, 573, IC-3,5, Saturnino de Brito St., Room 364, Campinas, São Paulo, 13083-852, Brazil.
School of Medical Sciences, University of Campinas, 126, Tessália Vieira de Camargo St., Campinas, São Paulo, 13083-887, Brazil.
Future Microbiol. 2023 Feb;18:197-203. doi: 10.2217/fmb-2022-0055. Epub 2023 Mar 14.
Ova and parasite examination by flotation requires hypertonic solutions, which can damage the egg and cyst membranes, leading to false negatives. The authors investigated the harmful effects of ZnSO and CHO solutions on the ova and parasite examination. The authors processed samples using the Three Fecal Test technique. Aliquots were floated in different pH levels, temperatures and solution densities. Densities above 1.12 g/ml led structures to collapse after 6-10 min. pH neutralization of the ZnSO solution did not prevent the parasites from changing. All structures were altered when standard methods were performed. To delay collapse, the parasite floating under 5 °C is highly desirable.
通过漂浮法进行的虫卵和寄生虫检查需要使用高渗溶液，这可能会损害虫卵和包囊膜，导致假阴性结果。作者研究了硫酸锌（ZnSO）溶液和蔗糖（CHO）溶液对虫卵和寄生虫检查的有害影响。作者采用三粪检技术处理样本。将等分试样漂浮在不同的pH值、温度和溶液密度条件下。密度高于1.12克/毫升会导致结构在6至10分钟后坍塌。硫酸锌溶液的pH值中和并不能阻止寄生虫发生变化。当采用标准方法时，所有结构都会改变。为了延迟坍塌，非常希望在5°C以下漂浮寄生虫。
