Hu Yaojie, Zhang Chunying, Chang Qing, Du Jinghai, Lu Haodong, Guo Xin, Chang Wang, Liu Shiguang, Chen Chunyou
Department of Head and Neck Surgery, Tangshan Gongren Hospital, Tangshan, Hebei, China.
Endokrynol Pol. 2023;74(2):144-152. doi: 10.5603/EP.a2023.0001. Epub 2023 Mar 14.
Thyroid cancer (TC) is a common endocrine malignancy, comprising nearly one-third of all head and neck malignancies worldwide. MicroRNAs (miRNAs) have been implicated in the malignant progression of multiple cancers; however, their contribution to thyroid diseases has not been fully explored.
This study aimed to illustrate the regulatory mechanism of microRNA-196a-5p in TC progression and to investigate whether microRNA-196a-5p affects progression of TC cells by targeting low-density lipoprotein receptor-associated protein 1B (LRP1B). MicroRNA-196a-5p and LRP1B expression status in TC cells and normal human thyroid cells was detected by quantative reverse transcription polymerase chain reaction (qRT-PCR) and western blot. Dual-luciferase reporter assay, cell counting kit-8 (CCK-8) assay, scratch healing assay, and Transwell assay were also performed.
The results showed that microRNA-196a-5p expression was up-regulated and LRP1B expression was down regulated in TC cells. In addition, the upregulation of microRNA-196a-5p facilitated progression of TC cells. Silencing microRNA-196a-5p led to the opposite results. Dual-luciferase reporter assay offered evidence for microRNA-196a-5p targeting LRP1B in TC. MicroRNA-196a-5p could target LRP1B to facilitate proliferation, invasion, and migration of TC cells.
Overall, this study revealed that microRNA-196a-5p may be a cancer-promoting microRNA that plays an important role in TC progression.
甲状腺癌（TC）是一种常见的内分泌恶性肿瘤，占全球所有头颈恶性肿瘤的近三分之一。微小RNA（miRNA）与多种癌症的恶性进展有关；然而，它们对甲状腺疾病的作用尚未得到充分研究。
本研究旨在阐明微小RNA-196a-5p在甲状腺癌进展中的调控机制，并研究微小RNA-196a-5p是否通过靶向低密度脂蛋白受体相关蛋白1B（LRP1B）影响甲状腺癌细胞的进展。通过定量逆转录聚合酶链反应（qRT-PCR）和蛋白质免疫印迹法检测甲状腺癌细胞和正常人甲状腺细胞中微小RNA-196a-5p和LRP1B的表达状态。还进行了双荧光素酶报告基因检测、细胞计数试剂盒-8（CCK-8）检测、划痕愈合检测和Transwell检测。
结果显示，甲状腺癌细胞中微小RNA-196a-5p表达上调，LRP1B表达下调。此外，微小RNA-196a-5p的上调促进了甲状腺癌细胞的进展。沉默微小RNA-196a-5p导致相反的结果。双荧光素酶报告基因检测为甲状腺癌中微小RNA-196a-5p靶向LRP1B提供了证据。微小RNA-196a-5p可靶向LRP1B促进甲状腺癌细胞的增殖、侵袭和迁移。
总体而言，本研究表明微小RNA-196a-5p可能是一种促进癌症的微小RNA，在甲状腺癌进展中起重要作用。
