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微小 RNA-873-5p 通过靶向 CXCL5 并调节 P53 通路抑制甲状腺癌细胞的恶性行为。

MicroRNA-873-5p suppresses cell malignant behaviors of thyroid cancer via targeting CXCL5 and regulating P53 pathway.

机构信息

Department of Head and Neck Surgery, Tangshan Gongren Hospital, Tangshan, Hebei Province, China.

出版信息

Hum Vaccin Immunother. 2022 Nov 30;18(5):2065837. doi: 10.1080/21645515.2022.2065837. Epub 2022 Apr 29.

DOI:10.1080/21645515.2022.2065837
PMID:35486941
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9302501/
Abstract

We aimed to examine the roles of microRNA-873-5p and CXCL5 in thyroid cancer (TC) cells. qRT-PCR was adopted to measure the expression levels of CXCL5 mRNA and microRNA-873-5p in TC cells, and western blot was adopted to evaluate the CXCL5 protein expression level. Bioinformatics analysis was done to predict the upstream gene of CXCL5. Dual-luciferase assay was applied to validate the binding relationship of CXCL5 and the upstream regulatory gene. Cell experiments were done to detect the effects of microRNA-873-5p targeting CXCL5 on malignant progression of cancer cells. Western blot was adopted to demonstrate the phosphorylation level of P53 pathway related-proteins. CXCL5 was upregulated in TC cells and tissues. The results of in vitro assays displayed that CXCL5 downregulation dramatically suppressed the malignant behaviors of TC cells. MicroRNA-873-5p suppressed CXCL5 expression, but the suppressive effect of microRNA-873-5p on TC cells was abolished through CXCL5 overexpression. Additionally, microRNA-873-5p could mediate p53 pathway and thereby inhibit the malignant behaviors of TC cells through targeting CXCL5. In summary, we proved that microRNA-873-5p repressed the malignant behaviors of TC cells through targeting CXCL5 and P53 pathway, indicating that microRNA-873-5p can be a biomarker for TC.

摘要

我们旨在研究 microRNA-873-5p 和 CXCL5 在甲状腺癌 (TC) 细胞中的作用。采用 qRT-PCR 检测 TC 细胞中 CXCL5 mRNA 和 microRNA-873-5p 的表达水平，采用 Western blot 检测 CXCL5 蛋白表达水平。采用生物信息学分析预测 CXCL5 的上游基因。应用双荧光素酶报告基因实验验证 CXCL5 与上游调控基因的结合关系。细胞实验检测 microRNA-873-5p 靶向 CXCL5 对癌细胞恶性进展的影响。采用 Western blot 检测 P53 通路相关蛋白的磷酸化水平。结果显示，CXCL5 在 TC 细胞和组织中上调。体外实验结果表明，CXCL5 下调显著抑制 TC 细胞的恶性行为。microRNA-873-5p 抑制 CXCL5 的表达，但通过 CXCL5 过表达，microRNA-873-5p 对 TC 细胞的抑制作用被消除。此外，microRNA-873-5p 可以通过靶向 CXCL5 介导 p53 通路，从而抑制 TC 细胞的恶性行为。综上所述，我们证明了 microRNA-873-5p 通过靶向 CXCL5 和 P53 通路抑制 TC 细胞的恶性行为，表明 microRNA-873-5p 可作为 TC 的生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9e/9302501/0240cb76ae99/KHVI_A_2065837_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9e/9302501/2b82efae62fb/KHVI_A_2065837_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9e/9302501/75432ffe7795/KHVI_A_2065837_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9e/9302501/b4d58444a858/KHVI_A_2065837_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9e/9302501/6a34a6115565/KHVI_A_2065837_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9e/9302501/0240cb76ae99/KHVI_A_2065837_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9e/9302501/2b82efae62fb/KHVI_A_2065837_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9e/9302501/75432ffe7795/KHVI_A_2065837_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9e/9302501/b4d58444a858/KHVI_A_2065837_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9e/9302501/6a34a6115565/KHVI_A_2065837_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f9e/9302501/0240cb76ae99/KHVI_A_2065837_F0005_OC.jpg

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