Xuzhou Institute of Agricultural Sciences of the Xuhuai District of Jiangsu Province, Xuzhou 221000, China; Tongshan Test Station, Xuzhou Institute of Agricultural Sciences of the Xuhuai District of Jiangsu Province, Xuzhou 221121, China.
Xuzhou Institute of Agricultural Sciences of the Xuhuai District of Jiangsu Province, Xuzhou 221000, China.
J Food Prot. 2023 Jan;86(1):100006. doi: 10.1016/j.jfp.2022.10.005. Epub 2022 Dec 16.
Imazalil is a chiral fungicide widely used to protect strawberries against gray mold, which may pose threats to food safety. This study aims to investigate the stereoselective behavior of imazalil during strawberry growth and strawberry wine production. A method was proposed and validated for the extraction and quantitative analysis of imazalil residues in strawberry, strawberry pomace, and strawberry wine by using ultra-high performance liquid chromatography-tandem mass spectrometry. The method exhibited mean recoveries ranging from 86.2% to 119.7% with relative standard deviations of 0.1-11.3%. The dissipation curve of imazalil during strawberry growth followed the first-order kinetic model with a half-life ranging from 6.5 to 7.1 days. Significant enantioselectivity of imazalil was observed in strawberry grown under field conditions and strawberry wine production process, with enantiomeric fraction values ranging from 0.51 (2 h) to 0.42 (27d) and from 0.48 (0d) to 0.52 (10d), respectively. (+)-imazalil was preferentially degraded in strawberry under field conditions, while (-)-imazalil was preferentially degraded during the fermentation process. The processing factor was lower than 1 for each procedure, indicating that the wine-making process can reduce imazalil residue in strawberry. These findings may facilitate a more accurate risk assessment of imazalil and provide important guidance for the safe and efficacious use of imazalil in agriculture.
抑霉唑是一种手性杀菌剂，广泛用于保护草莓免受灰霉病的侵害，但它可能对食品安全构成威胁。本研究旨在探讨抑霉唑在草莓生长和草莓酒生产过程中的立体选择性行为。建立了一种超高效液相色谱-串联质谱法用于草莓、草莓渣和草莓酒中抑霉唑残留的提取和定量分析。该方法的平均回收率在 86.2%至 119.7%之间，相对标准偏差在 0.1%至 11.3%之间。抑霉唑在草莓生长过程中的消解曲线符合一级动力学模型，半衰期为 6.5 至 7.1 天。在田间条件下生长的草莓和草莓酒生产过程中，抑霉唑表现出显著的对映体选择性，对映体分数值分别在 0.51（2 h）至 0.42（27 d）和 0.48（0 d）至 0.52（10 d）之间变化。（+）-抑霉唑在田间条件下优先降解，而（-）-抑霉唑在发酵过程中优先降解。每个过程的加工因子均小于 1，表明酿酒过程可以降低草莓中的抑霉唑残留。这些发现可能有助于更准确地评估抑霉唑的风险，并为农业中安全有效地使用抑霉唑提供重要指导。
