Sun Caixia, Cang Tao, Wang Zhiwei, Wang Xinquan, Yu Ruixian, Wang Qiang, Zhao Xueping
State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control, MOA Key Lab for Pesticide Residue Detection, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, 198# Shiqiao Road, Hangzhou, Zhejiang, People's Republic of China.
Environ Monit Assess. 2015 May;187(5):303. doi: 10.1007/s10661-015-4539-x. Epub 2015 Apr 30.
The health risk to humans of pesticide application on minor crops, such as strawberry, requires quantification. Here, the dissipation and residual levels of three fungicides (pyraclostrobin, myclobutanil, and difenoconazole) were studied for strawberry under greenhouse conditions using high-performance liquid chromatography (HPLC)-tandem mass spectrometry after Quick, Easy, Cheap, Effective, Rugged, and Safe extraction. This method was validated using blank samples, with all mean recoveries of these three fungicides exceeding 80%. The residues of all three fungicides dissipated following first-order kinetics. The half-lives of pyraclostrobin, myclobutanil, and difenoconazole were 1.69, 3.30, and 3.65 days following one time application and 1.73, 5.78, and 6.30 days following two times applications, respectively. Fungicide residue was determined by comparing the estimated daily intake of the three fungicides against the acceptable daily intake. The results indicate that the potential health risk of the three fungicides was not significant in strawberry when following good agricultural practices (GAP) under greenhouse conditions.
在草莓等小作物上施用农药对人类健康造成的风险需要进行量化。在此，采用快速、简便、廉价、高效、耐用和安全（QuEChERS）萃取法，利用高效液相色谱（HPLC）-串联质谱法，研究了温室条件下草莓中三种杀菌剂（吡唑醚菌酯、腈菌唑和苯醚甲环唑）的消解情况和残留水平。该方法通过空白样品进行了验证，这三种杀菌剂的平均回收率均超过80%。所有三种杀菌剂的残留均遵循一级动力学进行消解。吡唑醚菌酯、腈菌唑和苯醚甲环唑单次施用后的半衰期分别为1.69天、3.30天和3.65天，两次施用后的半衰期分别为1.73天、5.78天和6.30天。通过比较三种杀菌剂的估计每日摄入量与可接受每日摄入量来确定杀菌剂残留量。结果表明，在温室条件下遵循良好农业规范（GAP）时，这三种杀菌剂在草莓中的潜在健康风险并不显著。
