Grimes S, Weisz-Carrington P, Daum H, Smith J, Green L, Wright K, Stein G, Stein J
Research Service, Veterans Administration Medical Center, Shreveport, Louisiana 71130.
Exp Cell Res. 1987 Dec;173(2):534-45. doi: 10.1016/0014-4827(87)90293-x.
A rat histone H4 gene closely associated with the testis-specific H1t gene was isolated by screening the Sargent-Bonner rat genomic library using cloned human histone genes as probes. Both the H4 gene and the H1t gene are located on a 7-kb EcoRI genomic DNA fragment. Although the deduced amino acid sequence of the rat H4 histone is identical to that of the sequence of human histone H4, the nucleotide sequence of the coding region differs significantly from the coding region of the human H4 gene. Moreover, the relative spacing between the 5'-consensus sequence elements is unique for an H4 gene. S1-nuclease protection analyses reveal that both the H4 and H1t mRNA species are present in a fraction of rat testis cells highly enriched in pachytene spermatocytes, while only the H4 mRNA species is present in a rat myeloma cell line (Y3-Ag1.2.3). During a 1-h hydroxyurea treatment of the Y3 cells, which produces a 99% inhibition of DNA synthesis, the level of this H4 mRNA drops by only 50%, indicating that the stability of this mRNA is only partially coupled with DNA synthesis.
通过使用克隆的人组蛋白基因作为探针筛选萨金特 - 邦纳大鼠基因组文库，分离出了一个与睾丸特异性H1t基因紧密相关的大鼠组蛋白H4基因。H4基因和H1t基因都位于一个7kb的EcoRI基因组DNA片段上。尽管推导的大鼠H4组蛋白的氨基酸序列与人组蛋白H4的序列相同，但编码区的核苷酸序列与人H4基因的编码区有显著差异。此外，5' - 共有序列元件之间的相对间距对于H4基因来说是独特的。S1核酸酶保护分析表明，H4和H1t mRNA种类都存在于大鼠睾丸细胞的一部分中，这些细胞高度富集粗线期精母细胞，而在大鼠骨髓瘤细胞系（Y3 - Ag1.2.3）中只存在H4 mRNA种类。在用羟基脲处理Y3细胞1小时期间，DNA合成受到99%的抑制，这种H4 mRNA的水平仅下降50%，这表明该mRNA的稳定性仅部分与DNA合成相关。
