Drabent B, Kardalinou E, Bode C, Doenecke D
Abt. Molekularbiologie, Georg-August-Universität Göttingen, Germany.
DNA Cell Biol. 1995 Jul;14(7):591-7. doi: 10.1089/dna.1995.14.591.
Mouse and human H4 genes associated with the testis-specific H1t gene were isolated from genomic libraries and were sequenced. The deduced amino acid sequences are identical to other mouse or human H4 histones, but the genes differ significantly in their nucleotide sequences. Both the human and the mouse genes are located on the same DNA strand compared with the H1t gene. In contrast to this identical transcriptional orientation of H1t and its neighboring H4 gene in mouse and man, an H4 gene with the opposite orientation has been described in the vicinity of the rat H1t gene. Northern blot analysis of RNA from testicular cells separated by centrifugal elutriation, S1 nuclease mapping, and reverse transcriptase polymerase chain reaction (RT-PCR) amplification show that both the murine and human H4 genes, like the H1t gene, are expressed in testicular cells, whereas the H4 genes, in contrast to the H1t gene, are expressed in nontesticular human and mouse cell culture cells.
从基因组文库中分离出与睾丸特异性H1t基因相关的小鼠和人类H4基因，并进行测序。推导的氨基酸序列与其他小鼠或人类H4组蛋白相同，但这些基因的核苷酸序列存在显著差异。与H1t基因相比，人类和小鼠基因均位于同一条DNA链上。与小鼠和人类中H1t及其相邻H4基因相同的转录方向相反，在大鼠H1t基因附近描述了一个方向相反的H4基因。对通过离心淘析分离的睾丸细胞RNA进行Northern印迹分析、S1核酸酶作图和逆转录酶聚合酶链反应（RT-PCR）扩增表明，与H1t基因一样，小鼠和人类H4基因均在睾丸细胞中表达，而与H1t基因不同的是，H4基因在非睾丸的人类和小鼠细胞培养细胞中表达。
