Dr. K C Patel R & D Centre, Charotar University of Science & Technology, CHARUSAT Campus, Changa- 388 421, Anand, India.
P D Patel Institute of Applied Sciences, Charotar University of Science & Technology CHARUSAT Campus, Changa- 388 421, Anand, India.
J Mater Sci Mater Med. 2023 Mar 14;34(3):11. doi: 10.1007/s10856-023-06715-5.
The present study aimed to optimize magnetic fluid hyperthermia (MFH) protocols by standardizing MF incubation time, hyperthermic duration, magnetic field, and MFH sessions to achieve a better hyperthermic response for the profuse killing of human breast cancer cell cells MCF7. Magnetic nanoparticles and MF were characterized using XRD, VSM, and DLS. Induction heating was performed for 30 min at field strengths of 12.5 and 13.3 kA/m at a fixed frequency of 330 kHz with varying concentrations and incubation duration on MCF7 cells. Single and multiple sessions hyperthermia protocols were used to kill MCF7 cells and the cytotoxicity effect was analyzed using MTT assay. Single and multiple sessions MFH protocols were established to kill breast cancer cells utilizing 0.2 mg/mL MF at 13.3 kA/m field and 330 kHz frequency and maintaining the hyperthermic temperature of 43-45 °C for 30 min. The single session MFH revealed severe toxicity of MF leading to more than 75% of cell death after 24 h of MF incubation. Multiple sessions hyperthermia resulted in more than 90% killing of MCF7 cells after two consequent 3 h MF incubation with 3 h gap. Each 3 h of MF incubation was followed by 30 min of induction heating. Multiple sessions hyperthermia was effective in killing a larger cell population compared to the single session protocol. The results may help in optimizing protocols for the profuse killing of cancer cells of multiple origins, and aid in deciding futuristic in vivo MFH-based therapeutic strategies against breast cancer. Variation in MCF7 cells' viability due to HT, MF, and MF + HT in multiple sessions.
本研究旨在通过标准化 MF 孵育时间、热疗持续时间、磁场和 MFH 疗程来优化磁流体热疗 (MFH) 方案，以实现更好的热疗反应，从而大量杀死人乳腺癌细胞 MCF7。使用 XRD、VSM 和 DLS 对磁性纳米粒子和 MF 进行了表征。在 330 kHz 的固定频率下，在 12.5 和 13.3 kA/m 的场强下进行感应加热 30 分钟，同时改变 MCF7 细胞的浓度和孵育时间。使用单次和多次疗程 MFH 方案来杀死 MCF7 细胞，并使用 MTT 测定法分析细胞毒性作用。使用 13.3 kA/m 场和 330 kHz 频率的 0.2 mg/mL MF 建立单次和多次疗程 MFH 方案，将热疗温度维持在 43-45°C 30 分钟。单次疗程 MFH 显示 MF 具有严重的毒性，导致 MF 孵育 24 小时后超过 75%的细胞死亡。在两次连续的 3 小时 MF 孵育后，每个 3 小时的 MF 孵育间隔 3 小时，随后进行 30 分钟的感应加热，多次疗程热疗导致 MCF7 细胞的死亡率超过 90%。与单次疗程方案相比，多次疗程热疗更有效地杀死更大的细胞群体。这些结果可能有助于优化用于大量杀死多种来源癌细胞的方案，并有助于决定未来基于 MFH 的治疗策略在乳腺癌中的应用。由于 HT、MF 和 MF+HT 在多次疗程中，MCF7 细胞活力发生变化。
