Xing Yonghui, Liu Song, Tan Shuxin, Jiang Yi, Luo Xuesong, Hao Xiuli, Huang Qiaoyun, Chen Wenli
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, P. R. China.
Hubei Key Laboratory of Soil Environment and Pollution Remediation, Huazhong Agricultural University, Wuhan 430070, P. R. China.
Environ Sci Technol. 2023 Mar 28;57(12):4905-4914. doi: 10.1021/acs.est.3c00486. Epub 2023 Mar 14.
Microbial consortia have opened new avenues for heavy-metal remediation. However, the limited understanding of the overall effect of interspecific interactions on remediation efficacy hinders its application. Here, the effects of multispecies growth and biofilm formation on Cd immobilization were explored from direct and multiple interactions through random combinations of two or three rhizosphere bacteria. In monocultures, Cd stress resulted in an average decrease in planktonic biomass of 26%, but through cooperation, the decrease was attenuated in dual (21%) and triple cultures (13%), possibly involving an increase in surface polysaccharides. More than 65% of the co-cultures exhibited induction of biofilm formation under Cd stress, which further enhanced the role of biofilms in Cd immobilization. Notably, excellent biofilm-forming ability or extensive social induction makes and stand out in multispecies biofilm formation and Cd immobilization. These two core species significantly increase the colonization of soil microorganisms on rice roots compared to the control, resulting in a 40% decrease in Cd uptake by rice. Our study enhances the understanding of bacterial interactions under Cd stress and provides a novel strategy for adjusting beneficial soil consortia for heavy-metal remediation.
微生物群落为重金属修复开辟了新途径。然而，对种间相互作用对修复效果的整体影响了解有限，这阻碍了其应用。在此，通过两种或三种根际细菌的随机组合，从直接和多重相互作用方面探索了多物种生长和生物膜形成对镉固定的影响。在单培养中，镉胁迫导致浮游生物量平均下降26%，但通过合作，在双培养（21%）和三培养（13%）中下降幅度减弱，这可能与表面多糖增加有关。超过65%的共培养在镉胁迫下表现出生物膜形成的诱导，这进一步增强了生物膜在镉固定中的作用。值得注意的是，出色的生物膜形成能力或广泛的群体感应使[具体物种1]和[具体物种2]在多物种生物膜形成和镉固定方面脱颖而出。与对照相比，这两个核心物种显著增加了土壤微生物在水稻根上的定殖，导致水稻对镉的吸收减少40%。我们的研究增进了对镉胁迫下细菌相互作用的理解，并为调整有益土壤群落以进行重金属修复提供了新策略。
