Kamal Nehal, Qian Chen, Hao Huanhuan, Wu Juanzi, Liu Zhiwei, Zhong Xiaoxian, Ghanem Osama M, Salem Ali, Orban Zoltan, Elwakeel Abdallah Elshawadfy, Mahmoud Samy F, Said Alaa F
National Forage Breeding Innovation Base (JAAS), Nanjing, 210014, People's Republic of China.
Department of Botany and Microbiology, Faculty of Science, Suez University, P.O. Box: 43221, Suez, Egypt.
Arch Microbiol. 2025 Jan 9;207(2):30. doi: 10.1007/s00203-024-04228-5.
Researchers have reported that Bacillus megaterium BM18-2 reduces Cd toxicity in Hybrid Pennisetum, but understanding the interaction between plants and associated endophytes is crucial for understanding phytoremediation strategies under heavy metal stress. The current study aims to monitor the colonization patterns of GFP-labeled endophytic bacteria BM18-2 on Hybrid Pennisetum grass. Additionally, it will monitor Cd's effect on plant bacterial colonization. Confocal laser scanning microscopy of plant roots infected with gfp tagged BM18-2 revealed that the bacterium colonised root hairs and epidermal cells at the early stage of colonization, and over time, the bacteria penetrated to the internal tissues following their colonization of the stem and leaf. The roots, stems, and leaves of H. Pennisetum grown in Cd-contaminated soil contained a higher number of bacteria than those grown in normal soil. The result of Cd translocation indicated the condensation of heavy metals in the root cells and stem, while no Cd was found in the leaf. The study will also look for the enzymatic activity of bacteria BM18-2 and use Leadmium Green AM dye to track how Cd is taken up and moved through the plant. The enzymatic activity results showed that BM18-2 can produce catalase and amylase, but did not record any cellulase or lipase activity. As a result, the pattern of useful endophytic BM18-2 colonization through H. Pennisetum grass will aid in the application and maintenance of these bacteria in farming, and it presents new opportunities for the development of innovative strategies in the fields of agriculture and biotechnology.
研究人员报告称巨大芽孢杆菌BM18 - 2可降低杂交狼尾草中的镉毒性，但了解植物与相关内生菌之间的相互作用对于理解重金属胁迫下的植物修复策略至关重要。当前研究旨在监测绿色荧光蛋白标记的内生细菌BM18 - 2在杂交狼尾草上的定殖模式。此外，还将监测镉对植物细菌定殖的影响。对感染了绿色荧光蛋白标记的BM18 - 2的植物根系进行共聚焦激光扫描显微镜观察发现，该细菌在定殖早期定殖于根毛和表皮细胞，随着时间推移，在定殖于茎和叶之后，细菌穿透至内部组织。生长在镉污染土壤中的杂交狼尾草的根、茎和叶所含细菌数量高于生长在正常土壤中的植株。镉转运结果表明重金属在根细胞和茎中浓缩，而叶片中未发现镉。该研究还将探寻细菌BM18 - 2的酶活性，并使用铅绿素AM染料追踪镉在植物中的吸收和转运过程。酶活性结果显示BM18 - 2可产生过氧化氢酶和淀粉酶，但未检测到任何纤维素酶或脂肪酶活性。因此，有益内生菌BM18 - 2在杂交狼尾草中的定殖模式将有助于这些细菌在农业中的应用和维持，为农业和生物技术领域创新策略的发展提供新机遇。
