Physiology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Physiology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Can J Physiol Pharmacol. 2023 Jun 1;101(6):286-293. doi: 10.1139/cjpp-2022-0482. Epub 2023 Mar 14.
Myocardial infarction is a common cause of disability. Decorin is a myokine that has anti-inflammatory, anti-apoptotic effects. Some studies stated that decorin protects myocardium from ischemia. Other studies stated that decorin levels are associated with acute coronary syndrome. The study aimed to investigate the therapeutic role of decorin on cardiac function in a rat model of myocardial infarction. Thirty adult male Wistar rats were divided into control group-rats were subcutaneously injected with normal saline, isoprenaline-injected group-rats were subcutaneously injected with isoprenaline (85 mg/kg) once daily for 2 days to induce myocardial infarction, and decorin ± isoprenaline-injected group-rats were injected as the previous group, followed by decorin injection 0.1 mg/kg) once daily for 7 days. Cardiac hemodynamics, serum lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), oxidative stress markers, gene expression for myocardial-transforming growth factor beta 1 (TGF-β1), interleukin 1 b (IL-1β), tumor necrosis factor alpha (TNF-α), and cardiac caspase-3 immunohistochemical analysis were done. Isoprenaline + decorin group had significant improvement in cardiac hemodynamics and oxidative stress markers; significant decrease in serum CK-MB, LDH, and myocardial gene expression for TNF-α, IL-1β, and TGF-β1; and decreased cardiac caspase-3 immunoreactivity was present. Therefore, decorin can be used as a therapeutic agent after myocardial infarction as it improved the cardiac function.
心肌梗死是一种常见的致残原因。 核心蛋白聚糖是一种肌源因子，具有抗炎、抗细胞凋亡作用。一些研究表明核心蛋白聚糖可保护心肌免受缺血损伤。其他研究表明核心蛋白聚糖水平与急性冠状动脉综合征相关。本研究旨在探讨核心蛋白聚糖在心肌梗死大鼠模型中心脏功能的治疗作用。 30 只成年雄性 Wistar 大鼠分为对照组-皮下注射生理盐水；异丙肾上腺素注射组-皮下注射异丙肾上腺素（85mg/kg），每天一次，连续 2 天以诱导心肌梗死；核心蛋白聚糖±异丙肾上腺素注射组-如上所述注射，随后每天注射核心蛋白聚糖（0.1mg/kg），连续 7 天。进行心脏血液动力学、血清乳酸脱氢酶（LDH）、肌酸激酶同工酶-MB（CK-MB）、氧化应激标志物、心肌转化生长因子-β1（TGF-β1）、白细胞介素 1β（IL-1β）、肿瘤坏死因子-α（TNF-α）基因表达和心脏 caspase-3 免疫组织化学分析。异丙肾上腺素+核心蛋白聚糖组心脏血液动力学和氧化应激标志物显著改善；血清 CK-MB、LDH 和心肌 TNF-α、IL-1β 和 TGF-β1 基因表达显著降低；心脏 caspase-3 免疫反应性降低。因此，核心蛋白聚糖可作为心肌梗死后的治疗药物，可改善心脏功能。
