Department of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Dariyah, Riyadh 13713, Saudi Arabia.
Department of Clinical Laboratory Sciences, The Faculty of Applied Medical Sciences, Taif University, Taif 21944, Saudi Arabia.
Molecules. 2021 Aug 25;26(17):5137. doi: 10.3390/molecules26175137.
In isoprenaline (ISO)-induced myocardial infarcted rats, garlic oil (GO) and its main ingredient, diallyl disulfide (DADS), were examined for cardioprotective effects when used with carvedilol (CAR). GO, DADS and CAR were given to rats in their respective groups, either alone or together, with the addition of isoprenaline (3 mg/kg/day, subcutaneously) during the last 10 days of treatment. At the end of 14 days of treatment, blood samples were collected, the hearts were excised under anesthesia and weighed. Heart tissue homogenate was used to measure superoxide dismutase (SOD), catalase (CAT), and thiobarbituric acid reactive substances (TBARS). Furthermore, the serum activities of cardiac markers, including lactate dehydrogenase, creatine kinase, and cardiac troponin, were checked. Moreover, inflammatory markers including tumor necrosis factor alpha, interleukin one beta, interleukin six, and kappa bp65 subunit were assessed. Rats that received GO, DADS, and CAR exhibited a significant increase in the cardiac antioxidant enzyme activities with a simultaneous decrease in serum cardiac markers enzymes and inflammatory markers. The TBARS were significantly reduced in rats that received treatment. The addition of carvedilol to GO or DADS significantly elevated antioxidant activities and decreased the release of cardiac enzymes into blood circulation. Both DADS and GOl were almost similar in efficacy, indicating the potential role of DADS in garlic oil-mediated cardioprotection. Combining GO or DADS with CAR increased CAR's cardioprotective impact and protected rats from developing ISO-induced myocardial infarction.
在异丙肾上腺素（ISO）诱导的心肌梗死大鼠中，研究了大蒜油（GO）及其主要成分二烯丙基二硫化物（DADS）与卡维地洛（CAR）合用的心脏保护作用。GO、DADS 和 CAR 分别被给予各自组的大鼠，无论是单独使用还是与最后 10 天治疗期间的异丙肾上腺素（3mg/kg/天，皮下注射）一起使用。在治疗 14 天后，收集血液样本，在麻醉下切除心脏并称重。使用心脏组织匀浆测量超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和硫代巴比妥酸反应物质（TBARS）。此外，还检查了包括乳酸脱氢酶、肌酸激酶和心肌肌钙蛋白在内的心脏标志物的血清活性。此外，还评估了包括肿瘤坏死因子-α、白细胞介素 1β、白细胞介素 6 和 kappa bp65 亚基在内的炎症标志物。接受 GO、DADS 和 CAR 治疗的大鼠表现出心脏抗氧化酶活性显著增加，同时血清心脏标志物酶和炎症标志物减少。TBARS 在接受治疗的大鼠中显著减少。将卡维地洛加入 GO 或 DADS 中可显著提高抗氧化活性，并减少心脏酶向血液循环中的释放。DADS 和 GOl 的功效几乎相似，表明 DADS 在大蒜油介导的心脏保护中的潜在作用。将 GO 或 DADS 与 CAR 结合使用可增强 CAR 的心脏保护作用，并防止大鼠发生 ISO 诱导的心肌梗死。
