Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, MOE Key Laboratory of Molecular Biophysics, Wuhan 430074, China.
Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, MOE Key Laboratory of Molecular Biophysics, Wuhan 430074, China.
Food Chem. 2023 Aug 15;417:135810. doi: 10.1016/j.foodchem.2023.135810. Epub 2023 Mar 7.
The increasing demand for greener food production makes biocatalysts more desirable than traditional production approaches. One limiting factor for biocatalyst efficiency is the immobilization strategy. In this work, a novel immobilization method was developed with the tyrosine-tag crosslinking mechanism. The immobilization efficiency was further enhanced with ultrasound treatment. Such a strategy was proven to be efficient with food enzyme lipase, d-amino acid oxidase and glucose dehydrogenase when they were immobilized on macroporous resins, amino resins, epoxy resins, and multiwalled carbon nanotubes. For lipase, glucose dehydrogenase and d-amino acid oxidase, the immobilization yield on macroporous resins increased by 20.4%, 21.1% and 24.1%, respectively. In addition, the immobilized enzymes had enhanced reusability, with a high degree of activity (more than 85%) detected after six cycles. Furthermore, the enzyme electrochemical sensors constructed by enzyme crosslinking have higher sensitivity, with peak currents 4-8 times those of sensors with uncrosslinked enzymes. The enzyme immobilization strategy developed in this study paves the way for better application of biocatalysts in the food industry.
对绿色食品生产的需求不断增加，使得生物催化剂比传统生产方法更受欢迎。生物催化剂效率的一个限制因素是固定化策略。在这项工作中，开发了一种新型的固定化方法，采用酪氨酸标记交联机制。超声处理进一步提高了固定化效率。当将食品酶脂肪酶、D-氨基酸氧化酶和葡萄糖脱氢酶固定在大孔树脂、氨基树脂、环氧树脂和多壁碳纳米管上时，这种策略被证明是有效的。对于脂肪酶、葡萄糖脱氢酶和 D-氨基酸氧化酶，固定化收率分别提高了 20.4%、21.1%和 24.1%。此外，固定化酶具有增强的可重复使用性，在六次循环后检测到的活性（超过 85%）很高。此外，通过酶交联构建的酶电化学传感器具有更高的灵敏度，峰电流是未交联酶传感器的 4-8 倍。本研究中开发的酶固定化策略为生物催化剂在食品工业中的更好应用铺平了道路。
