De Ambrosis A, Ferrari N, Bonassi S, Vidali G
Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
FEBS Lett. 1987 Dec 10;225(1-2):120-2. doi: 10.1016/0014-5793(87)81142-0.
Nucleosomal repeat lengths of total chromatin, H4 histone and beta-DR genes have been measured in logarithmically growing HeLa cells. We have detected significant differences in nucleosomal spacing between inactive chromatin and chromatin regions actively engaged in transcription. These differences are also maintained in metaphase chromosomes at times when transcription ceases although a shortening in nucleosomal repeat length is observed in active and inactive chromatin. These observations support a model where DNA-core histone interactions are temporarily altered to allow selective remodelling of chromatin organization.
已在对数生长期的HeLa细胞中测量了总染色质、H4组蛋白和β-DR基因的核小体重复长度。我们检测到无活性染色质与活跃参与转录的染色质区域之间的核小体间距存在显著差异。尽管在活跃和无活性染色质中均观察到核小体重复长度缩短，但在转录停止时的中期染色体中，这些差异仍然存在。这些观察结果支持了一种模型，即DNA-核心组蛋白相互作用会暂时改变，以允许染色质组织进行选择性重塑。
