Zha Fei, Pang Rui, Huang Shixuan, Zhang Jumei, Wang Juan, Chen Moutong, Xue Liang, Ye Qinghua, Wu Shi, Yang Meiyan, Gu Qihui, Ding Yu, Wu Qingping, Zhang Hao
School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Guangdong Provincial Key Laboratory of Microbial Safety and Health, Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Microbiomics and Precision Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, Guangdong, China.
J Proteomics. 2023 May 15;279:104866. doi: 10.1016/j.jprot.2023.104866. Epub 2023 Mar 12.
Vibrio parahaemolyticus, a sea-born bacterial pathogen, is a primary inducement of food-borne gastroenteritis. Previous studies have shown that non-coding small RNA plays a vital role in the regulation of multiple biological processes in pathogenic bacteria, especially autoaggregation and growth competition. However, the inherent mechanisms have not yet to be fully understood. As important regulators in Vibrios, the involvement of Qrr sRNAs in V. parahaemolyticus is largely unknown. Here, we carried out the Qrr5 deletion mutant and utilized a proteomic method to describe global proteomic alterations in response to Qrr5 deletion. A total of 297 significantly expressed proteins were determined between the Qrr5 deletion mutant and wild-type strain, among which 137 proteins were upregulated and 160 proteins were downregulated. The upregulated proteins principally participated in membrane transporters and signal transcription, while the downregulated proteins participated in the two-component system and transcription factor binding. Notably, transcriptional regulator LysR, outer membrane protein OmpA, and conjugal transfer protein TraA-related proteins were upregulated, causing the promotion of autoaggregation ability and growth competition ability against E. coli. This study provides insights into the regulatory network of sRNA in this bacterium, which will facilitate further explorations of important biological processes in pathogenic bacteria. SIGNIFICANCE: sRNA Qrr5 is an important regulator involved in bacterial multiple physiological processes, including auto-aggregation and growth competition among food-borne pathogens Vibrio parahaemolyticus. Here, utilizing a TMT-labeling proteomic approach, we identified 137 proteins were upregulated and 160 proteins were downregulated between the Qrr5 deletion mutant and wild-type strain. The upregulated proteins were involved in membrane transporters and signal transcription, while downregulated proteins were involved in the two-component system and transcription factor binding. Moreover, the LysR, OmpA, and TraA proteins were significantly upregulated, causing the promotion of autoaggregation and commensal growth competition ability. The mechanism of how Qrr5 regulates the targeted genes remains unclarified and need great efforts to explore.
副溶血性弧菌是一种海洋源性细菌病原体，是食源性肠胃炎的主要诱因。先前的研究表明，非编码小RNA在病原菌多种生物学过程的调控中起着至关重要的作用，尤其是在自聚集和生长竞争方面。然而，其内在机制尚未完全明确。作为弧菌中的重要调节因子，Qrr sRNAs在副溶血性弧菌中的作用在很大程度上尚不清楚。在此，我们构建了Qrr5缺失突变体，并利用蛋白质组学方法描述了因Qrr5缺失而引起的整体蛋白质组变化。在Qrr5缺失突变体和野生型菌株之间共鉴定出297种显著表达的蛋白质，其中137种蛋白质上调，160种蛋白质下调。上调的蛋白质主要参与膜转运和信号转录，而下调的蛋白质则参与双组分系统和转录因子结合。值得注意的是，转录调节因子LysR、外膜蛋白OmpA以及接合转移蛋白TraA相关蛋白上调，导致自聚集能力增强以及对大肠杆菌生长竞争能力的提升。本研究为该细菌中sRNA的调控网络提供了见解，这将有助于进一步探索病原菌中的重要生物学过程。意义：sRNA Qrr5是参与细菌多种生理过程的重要调节因子，包括食源性病原体副溶血性弧菌的自聚集和生长竞争。在此，我们利用TMT标记蛋白质组学方法，鉴定出在Qrr5缺失突变体和野生型菌株之间有137种蛋白质上调，160种蛋白质下调。上调的蛋白质参与膜转运和信号转录，而下调的蛋白质参与双组分系统和转录因子结合。此外，LysR、OmpA和TraA蛋白显著上调，导致自聚集和共生生长竞争能力增强。Qrr5如何调控靶基因的机制仍不明确，有待深入探索。
