Zha Fei, Pang Rui, Huang Shixuan, Zhang Jumei, Wang Juan, Chen Moutong, Xue Liang, Ye Qinghua, Wu Shi, Yang Meiyan, Gu Qihui, Ding Yu, Zhang Hao, Wu Qingping
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Guangdong Provincial Key Laboratory of Microbial Safety and Health, Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Microbiomics and Precision Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
Microorganisms. 2022 Oct 21;10(10):2084. doi: 10.3390/microorganisms10102084.
Small non-coding RNAs (sRNAs) in bacteria are important regulatory molecules for controlling virulence. In Vibrio spp., Qrr sRNAs are critical for quorum-sensing pathways and regulating the release of some virulence factors. However, the detailed role of Qrr sRNAs in the virulence of Vibrio parahaemolyticus remains poorly understood. In this study, we identified a Vibrio sRNA Qrr5 that positively regulates cytotoxicity and adherence in Caco-2 cells by primarily regulating the T3SS1 gene cluster. A number of 185, 586, 355, and 74 differentially expressed genes (DEGs) detected at 0, 2, 4, and 6 h post-infection, respectively, were mainly associated with ABC transporters and two-component system pathways. The DEGs exhibited a dynamic change in expression at various time points post-infection owing to the deletion of Qrr5. Accordingly, 17 related genes were identified in the co-expression network, and their interaction with Qrr5 was determined based on weighted co-expression network analysis during infection. Taken together, our results provide a comprehensive transcriptome profile of V. parahaemolyticus during infection in Caco-2 cells.
细菌中的小非编码RNA（sRNA）是控制毒力的重要调节分子。在弧菌属中，Qrr sRNA对于群体感应途径和调节某些毒力因子的释放至关重要。然而，Qrr sRNA在副溶血性弧菌毒力中的具体作用仍知之甚少。在本研究中，我们鉴定了一种弧菌sRNA Qrr5，它主要通过调节T3SS1基因簇来正向调节Caco-2细胞中的细胞毒性和黏附。在感染后0、2、4和6小时分别检测到的185、586、355和74个差异表达基因（DEG）主要与ABC转运蛋白和双组分系统途径相关。由于Qrr5的缺失，DEG在感染后的不同时间点表现出表达的动态变化。因此，在共表达网络中鉴定出17个相关基因，并基于感染期间的加权共表达网络分析确定了它们与Qrr5的相互作用。综上所述，我们的结果提供了副溶血性弧菌在Caco-2细胞感染期间的全面转录组概况。
