Davis S J, Burgoyne L A
School of Biology, Flinders University, Bedford Park, South Australia.
FEBS Lett. 1987 Dec 21;226(1):88-90. doi: 10.1016/0014-5793(87)80556-2.
The disomal series generated by the digestion of chromatin with DNase I has been followed to its highest orders using Klenow end-labelling and field-inversion gel electrophoresis to maximise the resolution of large DNA fragments. The series is coherent to the 16N level and as such is incompatible with the most structurally acceptable coiling models. We propose that this is evidence for the general unsuitability of coiling models and is support for the existence of simple 'back-to-back' double-stranded structures within chromatin.
通过用DNA酶I消化染色质产生的离散序列，利用Klenow末端标记和场反转凝胶电泳对其最高级次进行了追踪，以最大限度地提高大DNA片段的分辨率。该序列在16N水平上是连贯的，因此与最符合结构的盘绕模型不相符。我们认为，这证明了盘绕模型普遍不合适，并支持染色质中存在简单的“背靠背”双链结构。
