Department of Biotechnology and Biosafety, Graduate School of Natural and Applied Science, Eskisehir Osmangazi University, 26040 Eskisehir, Turkey.
Microbiota Biotechnology Industry and Trade Inc Co., Organized Industrial Zone, 34. Street, 26110 Eskişehir, Turkey.
Lett Appl Microbiol. 2023 Mar 1;76(3). doi: 10.1093/lambio/ovad035.
Enterococci are commensals of the human intestinal tract. Their use as probiotics is supported by their ability to confer several health benefits and eliminate foodborne pathogens but is controversial due to the presence of virulence and antibiotic resistance traits. To use them as probiotics requires thorough research to establish their safety. Here, we sequenced the whole genome of a newly isolated Enterococcus durans MN187066 and used a suite of bioinformatics tools to analyze its beneficial probiotic traits as well as antimicrobial resistance and virulence genes. The whole genome had a length of 2 978 152 bp, and an average G + C content of 37.88%. The bopABCD genes involved in biofilm formation were annotated in the genome. However, further analysis showed that these genes are mostly helpful in strengthening their colonization and establishment in the gastrointestinal tract. Also, we identified secondary metabolite gene clusters and the bacteriocins Enterolysin A and Enterocin P. We also identified repUS15 and rep1 replicons and genes that were associated with antimicrobial resistance and virulence. Nevertheless, vancomycin resistance genes were not detected. Our results show that the Ent. durans strain MN187066 can be considered a nontoxigenic strain and produces beneficial metabolites that are critical for their success as probiotics.
肠球菌是人类肠道的共生菌。它们具有赋予多种健康益处和消除食源性病原体的能力，因此被用作益生菌，但其存在毒力和抗生素耐药性特征，这一用途存在争议。要将其用作益生菌，需要进行彻底的研究以确定其安全性。在这里，我们对新分离的肠球菌 durans MN187066 进行了全基因组测序，并使用一系列生物信息学工具来分析其有益的益生菌特性以及抗微生物药物耐药性和毒力基因。全基因组长度为 2,978,152bp，平均 G+C 含量为 37.88%。基因组中注释了参与生物膜形成的 bopABCD 基因。然而，进一步的分析表明，这些基因主要有助于它们在胃肠道中的定植和建立。此外，我们还鉴定了次级代谢物基因簇和细菌素 Enterolysin A 和 Enterocin P。我们还鉴定了与抗微生物药物耐药性和毒力相关的 repUS15 和 rep1 复制子和基因。然而，未检测到万古霉素耐药基因。我们的结果表明，Ent. durans 菌株 MN187066 可以被认为是非毒性菌株，并产生有益的代谢产物，这对于它们作为益生菌的成功至关重要。
