Tarek Nashwa, El-Gendy Ahmed O, Khairalla Ahmed S, Abdel-Fattah Medhat, Tawfik Eman, Azmy Ahmed F
Department of Botany and Microbiology, Faculty of Science, Beni-Suef University, Egypt.
Basic Science Department, Faculty of Oral and Dental medicine, Nahda University Beni-Suef (NUB), Beni.
Mol Biol Res Commun. 2022;11(3):143-153. doi: 10.22099/mbrc.2022.44088.1760.
species are a long-standing and non-pathogenic commensal bacterium, representing an important part of the normal. is a rarely isolated species from animals and humans, and it was a tiny constituent of human oral cavity and animal intestinal flora, as well as animal-derived foods, particularly dairy products. This study evaluated the security of our strain NT21 by using whole-genome sequencing (WGS), physicochemical features, and antimicrobial activity. The complete genomic of our strain NT21was sequenced and analyzed by using several bioinformatics tools to identify bacteriocin genes, virulence genes, antibiotic resistance genes, Crispr-Cas and pathogenicity islands. The results showed that our strain NT21 lacks the presence of virulence genes, pathogenicity islands, plasmids and has only two antibiotic resistance genes. On the other hand, it produces three bacteriocin-like inhibitory substances (Enterolysin A, P and L50a). It has six gene-encoded Crisper-Cas and one cluster Crispr-Cas gene. According to our findings, NT21 is a possible probiotic strain that is safe for both human and animal use.
[物种名称]是一种长期存在的非致病性共生细菌，是正常菌群的重要组成部分。它是一种很少从动物和人类中分离出来的物种，是人类口腔、动物肠道菌群以及动物源性食品（特别是乳制品）中的微量成分。本研究通过全基因组测序（WGS）、理化特性和抗菌活性评估了我们的NT21菌株的安全性。使用多种生物信息学工具对我们的NT21菌株的完整基因组进行测序和分析，以鉴定细菌素基因、毒力基因、抗生素抗性基因、CRISPR-Cas和致病岛。结果表明，我们的NT21菌株不存在毒力基因、致病岛、质粒，并且只有两个抗生素抗性基因。另一方面，它产生三种类细菌素抑制物质（肠溶素A、P和L50a）。它有六个基因编码的CRISPR-Cas和一个成簇的CRISPR-Cas基因。根据我们的研究结果，NT21是一种对人和动物使用都安全的潜在益生菌菌株。
