Chen Fuqian, Xia Xiaotong, Ye Dong, Li Ting, Huang Xinxin, Cai Chun, Zhu Chenchen, Lin Chaozhan, Deng Tao, Liu Fang
School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
Artemisinin Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
Anal Chem. 2023 Apr 4;95(13):5773-5779. doi: 10.1021/acs.analchem.3c00073. Epub 2023 Mar 15.
Luminol and its derivatives are extensively used as chemiluminogenic substrates in bioimaging and biochemical analysis. Luminol reagents can typically emit blue chemiluminescence (CL), whose wavelength is normally outside the most sensitive detection range of human naked eyes and most CL analyzers with silicon-based charge-coupled device (CCD) detectors. Development of luminol analogues with longer wavelength emission is thus attractive. Herein, four new phthalhydrazide CL probes () have been prepared through the derivatization of luminol. The most promising one, 5-(4-hydroxy-1,3-dioxoisoindolin-2-yl)-2,3-dihydrophthalazine-1,4-dione (), emits bright green CL upon oxidation and shows enhanced CL performance compared to its parent luminol. Bloodstain imaging, horseradish peroxidase (HRP)-based immunoassay, and the analysis of glucose/glucose oxidase reaction have been performed using the reagent. These results indicate that is a new chemiluminogenic luminol analogue with great potential in real analytical applications and will be an alternative to replace luminol in practical CL analysis.
鲁米诺及其衍生物在生物成像和生化分析中被广泛用作化学发光底物。鲁米诺试剂通常能发出蓝色化学发光（CL），其波长通常在人眼和大多数配备基于硅的电荷耦合器件（CCD）探测器的CL分析仪最敏感的检测范围之外。因此，开发具有更长波长发射的鲁米诺类似物具有吸引力。在此，通过鲁米诺的衍生化制备了四种新型邻苯二甲酰肼CL探针（）。最有前景的一种，即5-(4-羟基-1,3-二氧代异吲哚啉-2-基)-2,3-二氢酞嗪-1,4-二酮（），氧化时发出明亮的绿色CL，与其母体鲁米诺相比，CL性能有所增强。使用该试剂进行了血迹成像、基于辣根过氧化物酶（HRP）的免疫分析以及葡萄糖/葡萄糖氧化酶反应的分析。这些结果表明，是一种新型的化学发光鲁米诺类似物，在实际分析应用中具有巨大潜力，将成为实际CL分析中替代鲁米诺的选择。
