Kricka L J, Ji X, Thorpe G H, Edwards B, Voyta J, Bronstein I
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104, USA.
J Immunoassay. 1996 Feb;17(1):67-83. doi: 10.1080/01971529608005779.
The utility of 5-hydroxy-2, 3-dihydrophthalazine-1, 4-dione (HDP) as a co-substrate for the chemiluminescent detection of horseradish peroxidase was assessed. Several substituted aryl boronic acid derivatives (4-phenyl, 4-iodo) acted as potent enhancers of the peroxidase catalyzed reaction. Addition of chelating agents (EDTA) and surfactants (Tween-20 and [poly (vinylbenzyl)tributylphosphonium chloride-poly (vinylbenzyl) trioctylphosphonium chloride copolymer]) modulated background light emission and the intensity and duration of the signal from both HDP and luminol. However, HDP was found to be inferior to luminol in the peroxidase assay. Comparative studies revealed that at 500 amol of peroxidase the S/B was ten-fold higher using a commercial luminol-based signal reagent as compared with an HDP-EDTA-Tween-20 reagent (S/B t = 0 min 21.8 vs 1.7, S/B t = 10 min 17.8 vs 2.0).
评估了5-羟基-2,3-二氢酞嗪-1,4-二酮（HDP）作为辣根过氧化物酶化学发光检测共底物的效用。几种取代芳基硼酸衍生物（4-苯基、4-碘）作为过氧化物酶催化反应的有效增强剂。添加螯合剂（EDTA）和表面活性剂（吐温-20和[聚（乙烯基苄基）三丁基氯化铵-聚（乙烯基苄基）三辛基氯化铵共聚物]）可调节背景光发射以及来自HDP和鲁米诺的信号强度和持续时间。然而，在过氧化物酶测定中发现HDP不如鲁米诺。比较研究表明，在500 amol过氧化物酶时，使用基于商业鲁米诺的信号试剂的S/B比使用HDP-EDTA-吐温-20试剂高十倍（S/B t = 0分钟时为21.8对1.7，S/B t = 10分钟时为17.8对2.0）。
