Whisenton L R, Granger N A, Bollenbacher W E
Department of Biology, University of North Carolina at Chapel Hill 27514.
Mol Cell Endocrinol. 1987 Dec;54(2-3):171-8. doi: 10.1016/0303-7207(87)90154-7.
An in vitro method for investigating 20-hydroxyecdysone regulation of the corpora allata (CA) has been used to assess the kinetics of stimulation of precommitment day 3 fifth (V) larval instar Manduca sexta CA by 20-hydroxyecdysone. The synthesis of juvenile hormone (JH) I and III acids by 20-hydroxyecdysone-stimulated CA incubated as complexes with the brain-corpora cardiaca (Br-CC) increased similarly over time; the synthesis of JH III acid was greater than that of JH I acid. Maximal stimulation of the CA to synthesize both JH acids occurred when the Br-CC-CA were exposed to 20-hydroxyecdysone for 30-60 min. Following stimulation, the elevated rates of JH I and JH III acid synthesis remained unchanged over an 11 h incubation in the absence of the steroid hormone, suggesting that once stimulated by 20-hydroxyecdysone the CA biosynthetic response is persistent. These kinetics data provide insight into the means by which 20-hydroxyecdysone stimulates the CA via the Br-CC.
一种用于研究20-羟基蜕皮激素对咽侧体（CA）调控的体外方法，已被用于评估20-羟基蜕皮激素对预蛹期第3天的五龄（V）烟草天蛾幼虫CA的刺激动力学。20-羟基蜕皮激素刺激的CA与脑-心侧体（Br-CC）形成复合体进行孵育，其合成保幼激素（JH）I和III酸的量随时间的增加情况相似；JH III酸的合成量大于JH I酸。当Br-CC-CA暴露于20-羟基蜕皮激素30 - 60分钟时，CA合成两种JH酸的刺激达到最大。刺激后，在没有类固醇激素的情况下孵育11小时，JH I和JH III酸合成的升高速率保持不变，这表明一旦被20-羟基蜕皮激素刺激，CA的生物合成反应具有持续性。这些动力学数据为20-羟基蜕皮激素通过Br-CC刺激CA的方式提供了见解。
