Granger N A, Mitchell L J, Janzen W P, Bollenbacher W E
Mol Cell Endocrinol. 1984 Oct;37(3):349-58. doi: 10.1016/0303-7207(84)90105-9.
When an in vitro assay system and radioimmunoassays specific for juvenile hormones (JH) I and III were used to probe the effect of co-incubating pupal brains with last instar larval corpora allata (CA) from the tobacco hornworm, Manduca sexta, a selective activation of JH III synthesis by the CA was observed. This homolog-specific activation suggested the presence of an allatotropic factor for the synthesis of JH III (JH III ATF), and its presence was demonstrated by the ability of a postmicrosomal supernatant of a day 0 pupal brain homogenate to activate the CA in vitro in a dose-dependent manner. This moiety appears to be a protein, based on its heat lability and protease sensitivity, and has an apparent molecular size of 40 kD and an isoelectric point of 5.5 JH III ATF activity is localized in specific neural tissues of the day 0 pupa, the brain and first three abdominal ganglia, with the brain containing 4 times the activity in the ganglia. The existence of this factor suggests that JH III synthesis by the CA of Manduca is regulated by a neuropeptide.
当使用体外测定系统和针对保幼激素（JH）I和III的放射免疫测定法来探究将烟草天蛾（Manduca sexta）蛹脑与末龄幼虫咽侧体（CA）共同孵育的效果时，观察到CA对JH III合成有选择性激活作用。这种同源物特异性激活表明存在一种用于JH III合成的促咽侧体因子（JH III ATF），并且通过0日龄蛹脑匀浆的微粒体后上清液能够以剂量依赖性方式在体外激活CA这一能力证明了它的存在。基于其热不稳定性和蛋白酶敏感性，该部分似乎是一种蛋白质，其表观分子大小为40 kD，等电点为5.5。JH III ATF活性定位于0日龄蛹的特定神经组织，即脑和前三腹神经节，其中脑中的活性是神经节中的4倍。该因子的存在表明烟草天蛾的CA合成JH III受一种神经肽调节。
