UMR 152 Pharma Dev, Université de Toulouse, IRD, UPS, France.
Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Perú.
Chem Biodivers. 2023 Apr;20(4):e202201087. doi: 10.1002/cbdv.202201087. Epub 2023 Mar 24.
Endophytic fungi possess a versatile metabolism which is related to their ability to live in diverse ecological niches. While culturing under laboratory conditions, their metabolism is mainly influenced by the culture media, time of incubation and other physicochemical factors. In this study, we focused on the production of 3 thiodiketopiperazines (TDKPs) botryosulfuranols A-C produced by an endophytic strain of Cophinforma mamane isolated from the leaves of Bixa orellana L collected in the Peruvian Amazon. We studied the time-course production of botryosulfuranols A-C during 28 days and evaluated the variations in the production of secondary metabolites, including the TDKPs, produced by C. mamane in response to different culture media, light versus dark conditions and different incubation times. We observed a short time-frame production of botryosulfuranol C while its production was significantly affected by the light conditions and nutrients of the culture media. Botryosulfuranols A and B showed a similar production pattern and a similar response to culturing conditions. Molecular networking allowed us to detect three compounds related to TDKPs that will be the focus of future experiments.
内生真菌具有多样的代谢途径，这与其在不同生态位中生存的能力有关。在实验室条件下培养时，它们的代谢主要受培养基、孵育时间和其他物理化学因素的影响。在这项研究中，我们专注于 3 种硫二酮哌嗪（TDKPs）——博里硫烷醇 A-C 的生产，这些化合物是从在秘鲁亚马逊地区采集的 Bixa orellana L. 叶片中分离出的内生菌株 Cophinforma mamane 产生的。我们研究了博里硫烷醇 A-C 在 28 天内的时间进程生产情况，并评估了 C. mamane 对不同培养基、光照与黑暗条件和不同孵育时间的响应，其次生代谢产物（包括 TDKPs）产生的变化。我们观察到博里硫烷醇 C 的生产时间很短，而其产量明显受到光照条件和培养基营养成分的影响。博里硫烷醇 A 和 B 的生产模式相似，对培养条件的响应也相似。分子网络使我们能够检测到三种与 TDKPs 相关的化合物，它们将是未来实验的重点。
