López-Villatoro J M, Díaz-Marsá M, De la Torre-Luque A, MacDowell K S, Prittwitz C, Leza J C, Carrasco J L
Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, Spain.
Department of Psychiatry and Medical Psychology, Faculty of Medicine, UCM, Madrid, Spain.
World J Biol Psychiatry. 2023 Sep-Oct;24(7):587-594. doi: 10.1080/15622975.2023.2183254. Epub 2023 Mar 15.
This study is designed to search for aggrupation of inflammatory/oxidative biomarker alterations in borderline personality disorder (BPD) and their association with phenotypic features.
Inflammatory/nitrosative proteins were measures in plasma and peripheral blood mononuclear cells obtained from BPD patients. Patients were assessed on different clinical dimensions of BPD. Oxidative damage was tested by measuring TBARS, nitrites, catalase, GPx and SOD. Protein expression of IκBα, NFκB, iNOS, COX-2, PPARγ, Keap1, NQO1, Nrf2 and α7nAChR was also determined. Western blot and ELISA were used for measurements and a cluster analysis of inflammatory/oxidative biomarkers alterations was performed to investigate subgroups of patients with similar alterations and its relationship with clinical features of BPD.
69 patients were included in the study. Two inflammatory/nitrosative clusters of patients were found: Cluster 1 patients showed significantly higher levels of GPx, IκBα, keap1, NQO1, PPARγ, α7nAChR and Nrf2 than cluster 2 patients. These patients had significantly longer duration of illness, milder anxiety symptoms and lower prescription of antipsychotic drugs than cluster 2.
Two clusters of BPD patients according to the inflammatory/nitrosative profiles were identified. Cluster 1 had increased antioxidant and anti-inflammatory biomarkers and was characterised by greater chronicity of illness but less acute symptomatic severity.
本研究旨在探寻边缘型人格障碍（BPD）中炎症/氧化生物标志物改变的聚集情况及其与表型特征的关联。
对BPD患者的血浆和外周血单核细胞中的炎症/亚硝化蛋白进行检测。对患者在BPD的不同临床维度上进行评估。通过测量丙二醛（TBARS）、亚硝酸盐、过氧化氢酶、谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）来检测氧化损伤。还测定了IκBα、核因子κB（NFκB）、诱导型一氧化氮合酶（iNOS）、环氧化酶-2（COX-2）、过氧化物酶体增殖物激活受体γ（PPARγ）、 Kelch样环氧氯丙烷相关蛋白1（Keap1）、醌氧化还原酶1（NQO1）、核因子E2相关因子2（Nrf2）和α7烟碱型乙酰胆碱受体（α7nAChR）的蛋白表达。采用蛋白质印迹法和酶联免疫吸附测定法进行测量，并对炎症/氧化生物标志物的改变进行聚类分析，以研究具有相似改变的患者亚组及其与BPD临床特征的关系。
69例患者纳入本研究。发现了两组炎症/亚硝化患者聚类：聚类1患者的GPx、IκBα、Keap1、NQO1、PPARγ、α7nAChR和Nrf2水平显著高于聚类2患者。与聚类2患者相比，这些患者的病程显著更长，焦虑症状更轻，抗精神病药物处方量更低。
根据炎症/亚硝化特征确定了两组BPD患者。聚类1具有增加的抗氧化和抗炎生物标志物，其特征为病程更长但急性症状严重程度较低。
