Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou, P. R. China.
Technical Center of Xiamen Customs, Xiamen, P. R. China.
J Sep Sci. 2023 May;46(10):e2200875. doi: 10.1002/jssc.202200875. Epub 2023 Mar 19.
A method was established for the separation and determination of triadimefon and its metabolite triadimenol enantiomer residues in major complementary fruit puree for infants and young children (banana puree, pineapple puree, and grape puree) by supercritical fluid chromatography. After the samples were extracted with acetonitrile and purified with a solid phase extraction cartridge, Acquity Trefoil CEL2 chiral chromatographic column was adopted for separation, and gradient elution was conducted at the flow rate of 1.0 ml/min under the mobile phase of supercritical carbon dioxide - 0.5% ammonia methanol, the detection wavelength was 220 nm and quantification was conducted with the external standard method. The limits of quantitation of triadimefon and triadimenol enantiomers were both 0.05 mg/kg, the linear ranges were 0.5-50 mg/L, and the linear correlation coefficients were greater than 0.9993. The recoveries in the spiked samples at 0.05, 0.2, and 3.0 mg/kg were from 80.1 to 106%, and the relative standard deviation reached 3.3-7.6%. The method is efficient, rapid, reproducible, and environmentally friendly, enabling accurate analysis of pesticide enantiomers, which can detect the enantiomer residues of triadimefon and its metabolite triadimenol in major complementary fruit puree for infants and young children.
建立了超临界流体色谱法同时测定婴幼儿辅助果泥（香蕉泥、菠萝泥、葡萄泥）中三唑酮及其代谢产物三唑醇对映体残留的分析方法。样品经乙腈提取，固相萃取小柱净化后，采用 Acquity Trefoil CEL2 手性色谱柱，以超临界二氧化碳-0.5%氨甲醇为流动相，梯度洗脱，流速 1.0ml/min，检测波长 220nm，外标法定量。三唑酮和三唑醇对映体的定量限均为 0.05mg/kg，线性范围为 0.5-50mg/L，线性相关系数均大于 0.9993。在 0.05、0.2 和 3.0mg/kg 添加水平下的回收率为 80.1%-106%，相对标准偏差为 3.3%-7.6%。该方法高效、快速、重现性好、环境友好，能够准确分析农药对映体，可用于检测婴幼儿辅助果泥中三唑酮及其代谢产物三唑醇的对映体残留。
