Ma Yun-Feng, Gong Lang-Lang, Zhang Meng-Qi, Liu Xuan-Zheng, Guo Huan, Hull J Joe, Long Gui-Jun, Wang Hong, Dewer Youssef, Zhang Fan, He Ming, He Peng
National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, P. R. China.
Pest Management and Biocontrol Research Unit, US Arid Land Agricultural Research Center, USDA Agricultural Research Services, Maricopa, Arizona 85138 United States.
J Agric Food Chem. 2023 Mar 29;71(12):4789-4801. doi: 10.1021/acs.jafc.2c08488. Epub 2023 Mar 15.
Insects have evolved an extremely sensitive olfactory system that is essential for a series of physiological and behavioral activities. Some carboxylesterases (CCEs) comprise a major subfamily of odorant-degrading enzymes (ODEs) playing a crucial role in odorant signal inactivation to maintain the odorant receptor sensitivity. In this study, 93 CCEs were annotated in the genome of the German cockroach , a serious urban pest. Phylogenetic and digital tissue expression pattern analyses identified two antenna-enriched CCEs, and , as candidate ODEs. RNA interference (RNAi)-mediated knockdown of and resulted in partial anosmia with experimental insects exhibiting reduced attraction to ester volatile resources and slower olfactory responses than controls. Furthermore, enzymatic conversion of geranyl acetate by crude male antennal extracts from and RNAi insects was also significantly reduced. Our results provide evidence for CCE function in German cockroach olfaction and provide a basis for further exploring behavioral inhibitors that target olfactory-related CCEs.
昆虫进化出了极其灵敏的嗅觉系统，这对一系列生理和行为活动至关重要。一些羧酸酯酶（CCEs）构成了气味降解酶（ODEs）的一个主要亚家族，在气味信号失活以维持气味受体敏感性方面发挥着关键作用。在本研究中，在德国小蠊（一种严重的城市害虫）的基因组中注释了93种CCEs。系统发育和数字组织表达模式分析确定了两种在触角中高度富集的CCEs，即 和 ，作为候选ODEs。RNA干扰（RNAi）介导的 和 的敲低导致部分嗅觉丧失，实验昆虫对酯类挥发性物质资源的吸引力降低，嗅觉反应比对照组慢。此外，来自 和 RNAi昆虫的雄性触角粗提物对乙酸香叶酯的酶促转化也显著降低。我们的结果为CCE在德国小蠊嗅觉中的功能提供了证据，并为进一步探索靶向嗅觉相关CCEs的行为抑制剂提供了基础。
