Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Mol Biotechnol. 2023 Dec;65(12):2038-2048. doi: 10.1007/s12033-023-00692-4. Epub 2023 Mar 15.
Traditional Chinese medicine has been used to treat diabetic foot ulcer (DFU) for a long time. However, the underlying mechanism of Radix arnebiae seu lithospermi ointment (RAS-ointment) has not been revealed. Effects of RAS-ointment treatment were observed in DFU patients. The endogenous competitive RNA mechanism was constructed based on micro-array sequencing and bioinformatics analysis. RT-PCR was used to detected the expression of genes in DFU ulcerated skins and non-ulcerated skins. Dual luciferase and RT-PCR experiments were used to investigate the endogenous competitive RNA mechanism. Based on micro-array sequencing and bioinformatics analysis, we found that SNHG12/NFYC-AS1, hsa-miR-199a-5p and S100A8/S100A7/XDH might form an endogenous competitive RNA mechanism. RT-PCR assay shown that SNHG12, NFYC-AS1, S100A8, S100A7 and XDH were significantly up-regulated, while hsa-miR-199a-5p was significantly down-regulated in DFU ulcerated skins (N = 10) compared with non-ulcerated skins (N = 10). Dual luciferase and RT-PCR experiments showed that SNHG12 or NFYC-AS1 up-regulated the expression of S100A8, S100A7 and XDH by inhibiting hsa-miR-199a-5p in a direct binding way. After 35 days of RAS-ointment treatment, the wound healing of DFU patients was substantially improved and the expression of S100A7 and XDH were reduced expression in DFU patients. In addition, the monomer composition of RAS-ointment, 49070_FLUKA or auraptenol inhibited the expression of S100A7 and XDH in Te317.sk cells. In conclusion, RAS-ointment may be used as an adjunctive therapy for DFU patients.
中药治疗糖尿病足溃疡（DFU）已有很长的历史。然而，紫草软膏（RAS-ointment）的作用机制尚未阐明。观察了 RAS-ointment 治疗 DFU 患者的效果。基于微阵列测序和生物信息学分析构建了内源性竞争 RNA 机制。用 RT-PCR 检测了 DFU 溃疡皮肤和非溃疡皮肤中基因的表达。使用双荧光素酶和 RT-PCR 实验研究了内源性竞争 RNA 机制。基于微阵列测序和生物信息学分析，我们发现 SNHG12/NFYC-AS1、hsa-miR-199a-5p 和 S100A8/S100A7/XDH 可能形成内源性竞争 RNA 机制。RT-PCR 检测表明，与非溃疡皮肤（N=10）相比，DFU 溃疡皮肤（N=10）中 SNHG12、NFYC-AS1、S100A8、S100A7 和 XDH 显著上调，而 hsa-miR-199a-5p 显著下调。双荧光素酶和 RT-PCR 实验表明，SNHG12 或 NFYC-AS1 通过直接结合抑制 hsa-miR-199a-5p 而上调 S100A8、S100A7 和 XDH 的表达。RAS-ointment 治疗 35 天后，DFU 患者的伤口愈合明显改善，DFU 患者 S100A7 和 XDH 的表达减少。此外，RAS-ointment 的单体成分 49070_FLUKA 或 auraptenol 抑制了 Te317.sk 细胞中 S100A7 和 XDH 的表达。总之，RAS-ointment 可作为 DFU 患者的辅助治疗。
