新种，从柑橘根际土壤中分离得到。

sp. nov., isolated from citrus rhizosphere soil.

作者信息

Liu Guo-Hong, Narsing Rao Manik Prabhu, Liu Ding-Qi, Tang Rong, Chen Qian-Qian, Shi Huai, Liu Bo, Li Wen-Jun, Zhou Shun-Gui

机构信息

Fujian Academy of Agricultural Sciences, Agricultural Bio-resources Institute, Fuzhou, Fujian 350003, PR China.

Programa de Doctorado en Ciencias Aplicadas, Universidad Autónoma de Chile, Talca 3460000, Chile.

出版信息

Int J Syst Evol Microbiol. 2023 Mar;73(3). doi: 10.1099/ijsem.0.005753.

DOI:10.1099/ijsem.0.005753

PMID:36920836

Abstract

A Gram-stain-positive, rod-shaped and motile strain, designated FJAT-49705, was isolated from the citrus rhizosphere soil sample. Strain FJAT-49705 grew at 20-40 °C (optimum, 30 °C) and pH 6.0-11.0 (optimum, pH 7.0) with 0-5 % (w/v) NaCl (optimum, 2 %). Strain FJAT-49705 showed high 16S rRNA gene sequence similarity to '' FJAT-25496 (99.7 %) and FJAT-18043 (98.0 %). In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic trees (based on 71 bacterial single-copy genes), strain FJAT-49705 clustered with the members of the genus . MK-7 was the only isoprenoid quinone present. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The major fatty acids were anteiso-C and iso-C. The genomic DNA G+C content was 36.9 %. The average nucleotide identity (ANI) values between FJAT-49705 and '' FJAT-25496 and FJAT-18043 were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity. Based on the above results, strain FJAT-49705 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FJAT-49705 (=CCTCC AB 2019243= LMG 31580).

摘要

从柑橘根际土壤样品中分离出一株革兰氏阳性、杆状且具运动性的菌株，命名为FJAT-49705。菌株FJAT-49705在20-40℃（最适温度为30℃）和pH 6.0-11.0（最适pH为7.0）的条件下生长，NaCl浓度为0-5%（w/v）（最适浓度为2%）。菌株FJAT-49705的16S rRNA基因序列与FJAT-25496（99.7%）和FJAT-18043（98.0%）具有高度相似性。在系统发育树（基于16S rRNA基因序列）和系统基因组树（基于71个细菌单拷贝基因）中，菌株FJAT-49705与该属的成员聚类在一起。MK-7是唯一存在的类异戊二烯醌。主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的磷脂。主要脂肪酸为anteiso-C和iso-C。基因组DNA的G+C含量为36.9%。FJAT-49705与FJAT-25496和FJAT-18043之间的平均核苷酸同一性（ANI）值低于作为种间同一性ANI标准推荐的截止水平（95-96%）。基于上述结果，菌株FJAT-49705代表该属的一个新物种，为此提出新物种名称sp. nov.。模式菌株为FJAT-49705（=CCTCC AB 2019243= LMG 31580）。