Liu Guo-Hong, Narsing Rao Manik Prabhu, Chen Qian-Qian, Shi Huai, Che Jian-Mei, Liu Bo, Li Wen-Jun
Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, Fujian, People's Republic of China.
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Arch Microbiol. 2022 Apr 25;204(5):281. doi: 10.1007/s00203-022-02886-x.
Two Gram-staining positive strains, FJAT-49825 and FJAT-50051 were isolated from a citrus rhizosphere soil sample. Strains FJAT-49825 and FJAT-50051 showed the highest 16S rRNA gene sequence similarity with the type strain of Neobacillus cucumis (98.4-98.5%). The 16S rRNA gene sequence similarity between strains FJAT-49825 and FJAT-50051 was 99.8%. Strain FJAT-49825 optimally grew at 35 °C, pH 6.0 in the absence of NaCl while strain FJAT-50051 grew at 40 °C, pH 7.0 and in presence of 2% NaCl (w/v). Both strains contained meso-2,6-diaminopimelic acid as the cell-wall diamino acid. The respiratory quinone of strains FJAT-49825 and FJAT-50051 was MK-7. The polar lipids of strain FJAT-49825 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified aminolipid and unidentified lipid whereas strain FJAT-50051 polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified phospholipid and unidentified lipid. The major fatty acids (> 10%) in both strains were iso-C and anteiso-C. The genomic DNA G + C content of strains FJAT-49825 and FJAT-50051 were 40.8 and 41.1%, respectively. The average nucleotide identity and digital DNA-DNA hybridization value between strains FJAT-49825 and FJAT-50051 and with other members of the genus Neobacillus were lower than the cut-off value (95-96/70%) for interspecies identity. Based on the results, strains FJAT-49825 and FJAT-50051 represent two novel species of the genus Neobacillus, for which the names Neobacillus rhizophilus sp. nov. and Neobacillus citreus sp. nov. are proposed. The type strains are FJAT-49825 (= GDMCC 1.2592 = JCM 34834) and FJAT-50051 (= GDMCC 1.2593 = JCM 34835).
从柑橘根际土壤样品中分离出两株革兰氏染色阳性菌株，FJAT-49825和FJAT-50051。菌株FJAT-49825和FJAT-50051与黄瓜新芽孢杆菌模式菌株的16S rRNA基因序列相似性最高（98.4 - 98.5%）。菌株FJAT-49825和FJAT-50051之间的16S rRNA基因序列相似性为99.8%。菌株FJAT-49825在35℃、pH 6.0且无NaCl的条件下生长最佳，而菌株FJAT-50051在40℃、pH 7.0且含有2% NaCl（w/v）的条件下生长。两株菌株均含有内消旋-2,6-二氨基庚二酸作为细胞壁二氨基酸。菌株FJAT-49825和FJAT-50051的呼吸醌为MK-7。菌株FJAT-49825的极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、未鉴定的氨基脂和未鉴定的脂，而菌株FJAT-50051的极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、未鉴定的磷脂和未鉴定的脂。两株菌株中主要脂肪酸（>10%）为异-C和anteiso-C。菌株FJAT-49825和FJAT-50051的基因组DNA G+C含量分别为40.8%和41.1%。菌株FJAT-49825和FJAT-50051与新芽孢杆菌属其他成员之间的平均核苷酸同一性和数字DNA-DNA杂交值低于种间同一性的阈值（95 - 96/70%）。基于这些结果，菌株FJAT-49825和FJAT-50051代表新芽孢杆菌属的两个新物种，为此提出了根际新芽孢杆菌新种（Neobacillus rhizophilus sp. nov.）和柑橘新芽孢杆菌新种（Neobacillus citreus sp. nov.）。模式菌株分别为FJAT-49825（=GDMCC 1.XXX = JCM 34834）和FJAT-50051（=GDMCC 1.XXX = JCM 34835）。 （注：原文中GDMCC和JCM编号部分未完整给出数字，翻译时保留原文格式）
