活细胞中的 DNA 标记 可见光诱导 [2+2] 光环加成反应。
DNA labelling in live cells visible light-induced [2+2] photocycloaddition.
机构信息
Institute of Organic Chemistry, Karlsruhe Institute of Technology (KIT), Fritz-Haber Weg 6, Karlsruhe 76131, Germany.
School of Chemistry and Physics, Centre for Materials Science, Queensland University of Technology (QUT), 2 George St, Brisbane QLD 4000, Australia.
出版信息
Chem Commun (Camb). 2023 Mar 30;59(27):4012-4015. doi: 10.1039/d3cc00817g.
We introduce a visible light-driven ( = 451 nm) photo-chemical strategy for labelling of DNA in living HeLa cells the [2+2] cycloaddition of a styrylquinoxaline moiety, which we incorporate into both the DNA and the fluorescent label. Our methodology offers advanced opportunities for the mild remote labelling of DNA in water while avoiding UV light activation.
我们介绍了一种可见光驱动（ = 451nm）的光化学策略，用于在活的 HeLa 细胞中标记 DNA——将一个苯乙烯喹喔啉部分进行 [2+2]环加成，我们将其同时引入 DNA 和荧光标记物中。我们的方法为在水中温和远程标记 DNA 提供了先进的机会，同时避免了 UV 光激活。
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