绒毛细胞提取物对肠道细胞增殖的抑制作用。
Inhibition of intestinal cell proliferation by villous cell extract.
作者信息
Rampal P, Nano J L, Zunino C
机构信息
Laboratoire d'Hépato-Gastro-Entérologie Faculté de Médecine, Nice, France.
出版信息
Gut. 1987;28 Suppl(Suppl):109-15. doi: 10.1136/gut.28.suppl.109.
In order to verify the hypothesis that intestinal cell proliferation is controlled by a mitotic inhibitor, extracts of villous epithelial cells from different species were analysed to study their effect on the proliferation of various intestinal cells. Villous extracts from rat and rabbit strongly and reversibly inhibited cell division and DNA synthesis in a rat intestinal epithelial cell line and a primary culture of rabbit intestinal epithelial cells. This non-cytotoxic, tissue specific but not species specific factor is present in both villous cells and crypt cells, with the highest concentrations occurring in the superficial epithelial cells. Assay of a partial purification of this factor showed that it has a molecular weight of approximately 190,000 daltons.
为了验证肠道细胞增殖受有丝分裂抑制剂控制这一假说，对来自不同物种的绒毛上皮细胞提取物进行了分析，以研究其对各种肠道细胞增殖的影响。大鼠和兔子的绒毛提取物强烈且可逆地抑制了大鼠肠道上皮细胞系和兔肠道上皮细胞原代培养物中的细胞分裂和DNA合成。这种无细胞毒性、组织特异性但非物种特异性的因子存在于绒毛细胞和隐窝细胞中，在表层上皮细胞中的浓度最高。对该因子部分纯化后的分析表明，其分子量约为190,000道尔顿。
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