Fukamachi H, Ichinose M, Tsukada S, Kakei N, Suzuki T, Miki K, Kurokawa K, Masui T
Zoological Institute, Faculty of Science, Tokyo, Japan.
Biochem Biophys Res Commun. 1994 Dec 15;205(2):1445-51. doi: 10.1006/bbrc.1994.2827.
Hepatocyte growth factor (HGF) stimulated the growth of fetal rat gastrointestinal epithelial cells in primary culture with a clear dose-response relationship. The epithelial response to the mitogenic activity of HGF was different among the region of the gastro-intestinal tract; glandular stomach most responsive, followed by intestine and forestomach. The interaction of HGF with other growth factors in inducing the epithelial growth was also different depending on the type of the epithelial cells, indicating a region-specific growth regulation in the gastro-intestinal tract. Analyses using Northern blot and RT-PCR revealed that HGF mRNA was expressed only in mesenchymes but not in epithelia of the gastro-intestinal tract while expression of c-met (HGF receptor) gene was observed in both tissues. These results suggest that gastro-intestinal mesenchymes secrete HGF which stimulates the growth of attaching epithelial cells by a paracrine mechanism, and that the epithelial response to HGF is controlled by a region-specific growth regulatory mechanism.
肝细胞生长因子（HGF）刺激原代培养的胎鼠胃肠上皮细胞生长，呈现明显的剂量反应关系。胃肠道不同区域的上皮细胞对HGF促有丝分裂活性的反应不同；腺胃反应最为敏感，其次是小肠和前胃。HGF与其他生长因子在诱导上皮细胞生长方面的相互作用也因上皮细胞类型而异，表明胃肠道存在区域特异性生长调节。Northern印迹和逆转录聚合酶链反应（RT-PCR）分析显示，HGF mRNA仅在胃肠道的间充质中表达，而在上皮中不表达，而c-met（HGF受体）基因在这两种组织中均有表达。这些结果表明，胃肠道间充质分泌HGF，通过旁分泌机制刺激附着的上皮细胞生长，并且上皮细胞对HGF的反应受区域特异性生长调节机制控制。
