Goodlad R A, Plumb J A, Wright N A
Department of Histopathology, Royal Postgraduate Medical School, Hammersmith Hospital, London.
Gut. 1987;28 Suppl(Suppl):189-92. doi: 10.1136/gut.28.suppl.189.
Intestinal cell proliferation and cell production is best quantified by measuring the rate of accumulation of vincristine arrested metaphases in microdissected intestinal crypts to determine the crypt cell production rate (CCPR). Studies of intestinal adaptation could be much more informative if a valid measure of intestinal function could also be included. One such method is the water absorption capacity. The CCPR of the jejunum and intestinal water absorption were measured in 19 groups of hypo and hyperproliferative rats which were in a 'steady state' of cell production and turnover. The minimum values were obtained after hypophysectomy and the maximum values were observed in lactation. Crypt cell production rate and absorption were significantly correlated (p less than 0.001) to each other. There was a significant (p less than 0.001) correlation between both CCPR and absorption and dry weight of the intestinal segment studies and food intake. Body weight was a poor predictor of either CCPR or absorption. The combined study of CCPR and water absorption is thus a practical and convenient approach to the study of intestinal cell proliferation and intestinal adaptation.
通过测量在显微解剖的肠隐窝中长春新碱阻滞的中期相的积累速率来确定隐窝细胞产生率（CCPR），这是对肠细胞增殖和细胞产生进行最佳量化的方法。如果还能纳入一种有效的肠功能测量方法，那么对肠道适应性的研究可能会提供更多信息。一种这样的方法是吸水能力。在处于细胞产生和周转“稳定状态”的19组低增殖和高增殖大鼠中，测量了空肠的CCPR和肠道吸水情况。垂体切除术后获得最小值，哺乳期观察到最大值。隐窝细胞产生率和吸收率彼此显著相关（p小于0.001）。CCPR和吸收率与所研究肠段的干重以及食物摄入量之间均存在显著（p小于0.001）相关性。体重对于CCPR或吸收率而言都是一个较差的预测指标。因此，CCPR和吸水的联合研究是研究肠细胞增殖和肠道适应性的一种实用且便捷的方法。
