探究肠道微环境中宿主-细菌相互作用的工具：从分子到细胞水平。
Tools for probing host-bacteria interactions in the gut microenvironment: From molecular to cellular levels.
机构信息
Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, United States.
Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, United States.
出版信息
Bioorg Med Chem Lett. 2020 May 15;30(10):127116. doi: 10.1016/j.bmcl.2020.127116. Epub 2020 Mar 18.
Healthy function of the gut microenvironment is dependent on complex interactions between the bacteria of the microbiome, epithelial and immune (host) cells, and the surrounding tissue. Misregulation of these interactions is implicated in disease. A range of tools have been developed to study these interactions, from mechanistic studies to therapeutic evaluation. In this Digest, we highlight select tools at the cellular and molecular level for probing specific cell-microenvironment interactions. Approaches are overviewed for controlling and probing cell-cell interactions, from transwell and microfluidic devices to engineered bacterial peptidoglycan fragments, and cell-matrix interactions, from three-dimensional scaffolds to chemical handles for in situ modifications.
肠道微环境的健康功能依赖于微生物组的细菌、上皮细胞和免疫（宿主）细胞以及周围组织之间的复杂相互作用。这些相互作用的失调与疾病有关。已经开发出一系列工具来研究这些相互作用，从机制研究到治疗评估。在这篇综述中，我们重点介绍了用于探测特定细胞-微环境相互作用的细胞和分子水平上的精选工具。从 Transwell 和微流控设备到工程化的细菌肽聚糖片段，以及从三维支架到用于原位修饰的化学接头，我们概述了用于控制和探测细胞-细胞相互作用的方法，以及用于探测细胞-基质相互作用的方法。
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