一种包覆在磁性纳米颗粒表面的人工蛋白质冠层：标记抗体的简单有效方法。（注：原文中“artificialed”拼写错误，应为“artificial”）

An artificialed protein corona coating the surface of magnetic nanoparicles:a simple and efficient method for label antibody.

作者信息

Zhao Penghua, Huang Xiaoyan, Li Yaping, Huo Xueping, Feng Qing, Zhao Xiangrong, Xu Cuixiang, Wang Jianhua

机构信息

Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, 710068, China.

Shaanxi Provincial Key Laboratory of Infection and Immune Diseases, Shaanxi Provincial People's Hospital, Xi'an, 710068, China.

出版信息

Heliyon. 2023 Mar 1;9(3):e13860. doi: 10.1016/j.heliyon.2023.e13860. eCollection 2023 Mar.

DOI:10.1016/j.heliyon.2023.e13860

PMID:36923872

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10008981/

Abstract

BACKGROUND

Protein Corona (PC) of nanoparticles is a structure which composed of one or more layers of proteins adsorbed on the surface of nanomaterials, and the formation of PC is a universal process of spontaneous randomness. We take advantage of the formation principle of the PC, developed a simple and efficient method for label protein to nanoparticles.

METHODS

The artificialed protein corona (APC) on the surface of nanoparticles was synthesized via the artificialed methods of desolvation aggregation and crosslinking with control.

RESULTS

The dosage of precipitator and the ratio of protein to magnetic nanoparticles (MNPs)(particle size: 3 nm) were optimized, and the core-shell nanoparticles with narrow particle size (particle size: 10 nm) distribution were obtained. The MNPs with APC were characterized by transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). Additionally, a hemolysis test on prepared MNPs was conducted with APC. The presence of APC coating on the surface of MNPs showed an improving effect to reduce the cytotoxicity. Cellular toxicity of MNPs with APC was also investigated on HFF1 cell lines. And the cells survival in the presence of APC coated MNPs and display neither reduced metabolism nor cytostatic effect. The functional test of the MNPs with APC showed that proteins can be modified and labeled onto magnetic nanoparticles and retain their original activity.

CONCLUSIONS

This marking method is gentle and effective. And the properties of the APC propose MNPs as a promising candidate for multifunctional biomedical applications.

摘要

背景

纳米颗粒的蛋白质冠层（PC）是一种由吸附在纳米材料表面的一层或多层蛋白质组成的结构，PC的形成是一个普遍的自发随机过程。我们利用PC的形成原理，开发了一种简单有效的将蛋白质标记到纳米颗粒上的方法。

方法

通过去溶剂化聚集和交联的人工方法并设置对照，合成纳米颗粒表面的人工蛋白质冠层（APC）。

结果

优化了沉淀剂的用量以及蛋白质与磁性纳米颗粒（MNPs，粒径：3nm）的比例，获得了粒径分布窄（粒径：10nm）的核壳纳米颗粒。用透射电子显微镜（TEM）和振动样品磁强计（VSM）对带有APC的MNPs进行了表征。此外，对制备的带有APC的MNPs进行了溶血试验。MNPs表面存在APC涂层显示出降低细胞毒性的改善作用。还在HFF1细胞系上研究了带有APC的MNPs的细胞毒性。并且在存在APC包被的MNPs的情况下细胞存活，既没有代谢降低也没有细胞抑制作用。对带有APC的MNPs的功能测试表明，蛋白质可以被修饰并标记到磁性纳米颗粒上并保留其原始活性。