Department of pharmaceutical biomaterials, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
Zanjan Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
Int J Biol Macromol. 2018 Mar;108:909-915. doi: 10.1016/j.ijbiomac.2017.10.180. Epub 2017 Oct 31.
In this study, iron oxide magnetic bovine serum albumin core-shell nanoparticles (BSA coated IONPs) with narrow particle size distribution were synthesized under one-pot reaction via the desolvation and chemical co-precipitation method. Functionalized IONPs were characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM) techniques. Furthermore, vibrating sample magnetometer (VSM) analysis show these nanoparticles (NPs) have an excellent magnetic properties. Cellular toxicity of IONPs was also investigated on HFF2 cell lines. Additionally, a hemolysis test of as prepared core-shell NPs were performed. The presence of albumin as a biomolecule coating on the surface of IONPs showed an improving effect to reduce the cytotoxicity. The properties of the designed NPs propose the BSA coated IONPs as a promising candidate for multifunctional biomedical applications.
在这项研究中，通过去溶剂化和化学共沉淀法，在一锅反应中合成了具有窄粒径分布的氧化铁磁性牛血清白蛋白核壳纳米粒子（BSA 包覆的 IONPs）。功能化的 IONPs 通过 X 射线衍射（XRD）、差示扫描量热法（DSC）、热重分析（TGA）、傅里叶变换红外光谱（FTIR）和透射电子显微镜（TEM）技术进行了表征。此外，振动样品磁强计（VSM）分析表明，这些纳米粒子（NPs）具有优异的磁性。还在 HFF2 细胞系上研究了 IONPs 的细胞毒性。此外，还对制备的核壳 NPs 进行了溶血试验。表面包覆白蛋白作为生物分子，对降低细胞毒性有显著的改善效果。设计的 NPs 的特性表明，BSA 包覆的 IONPs 是多功能生物医学应用的有前途的候选材料。
