Institute of STD/AIDS Prevention and Control, Beijing Center for Disease Prevention and Control, Beijing, China.
School of Public Health, China Medical University, Shenyang, China.
AIDS Res Hum Retroviruses. 2023 Aug;39(8):436-441. doi: 10.1089/AID.2022.0164. Epub 2023 Jun 13.
Two HIV-1 infections with unassigned genotypes were identified during HIV-1 pretreatment drug resistance surveillance. The near full-length genome sequences of BL5040-00 and BL5085-00 were obtained and were classified as unique recombinant forms (URFs) between CRF01_AE and CRF07_BC. Simplot (version 3.5) analyses showed that the two URFs shared similar recombinant forms, and in the backbone belonging to CRF01_AE, the , , , and gene fragments were genetically substituted by CRF07_BC. BL5040-00, with 10 breakpoints, had 6 CRF07_BC fragments and 5 CRF01_AE fragments, whereas BL5085-00, with 6 breakpoints, had 4 CRF07_BC fragments and 3 CRF01_AE fragments. BL5040-00 strain had two additional recombination breakpoints in - gene. The presence of URFs suggests that the men who have sex with men population in Beijing has an active HIV epidemic and the genetic diversity of HIV-1 is complex, emphasizing molecular epidemiology and disease progression monitoring should be strengthened.
在 HIV-1 治疗前耐药性监测中发现了两例未分配基因型的 HIV-1 感染。获得了 BL5040-00 和 BL5085-00 的近乎全长基因组序列，并将其分类为 CRF01_AE 和 CRF07_BC 之间的独特重组形式 (URF)。Simplot（版本 3.5）分析表明，这两种 URF 具有相似的重组形式，在属于 CRF01_AE 的骨架中， 、 、 、 和 基因片段被 CRF07_BC 取代。BL5040-00 有 10 个断点，有 6 个 CRF07_BC 片段和 5 个 CRF01_AE 片段，而 BL5085-00 有 6 个断点，有 4 个 CRF07_BC 片段和 3 个 CRF01_AE 片段。BL5040-00 株在 - 基因中有两个额外的重组断点。URF 的存在表明，北京的男男性行为人群中存在活跃的 HIV 流行，HIV-1 的遗传多样性很复杂，强调应加强分子流行病学和疾病进展监测。
