Department of Chemistry, School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan.
Laboratory for Chemistry and Life Science, Institute of innovative research, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8503, Japan.
J Phys Chem Lett. 2023 Mar 23;14(11):2886-2890. doi: 10.1021/acs.jpclett.2c03366. Epub 2023 Mar 16.
Silver and silver ions have a long history of antimicrobial activity and medical applications. Nevertheless, the activity of Ag against bacteria, how it enters a cell, has not yet been established. The K channel, a membrane protein, is a possible route. The addition of a channel inhibitor (4-aminopyridine) to modulate the Ag uptake could support this view. However, the inhibitor enhances the uptake of Ag, the opposite result. We have applied cold ion trap infrared laser spectroscopy to complexes of Ag and Ac-Tyr-NHMe (a model for GYG) which is a portion of the selectivity filter in the K channel to consider the question of permeation. With support from quantum chemical calculations, we have determined the stable conformations of the complex. The conformations strongly suggest that Ag would not readily permeate the K channel. The mechanism of the unexpected enhancement by the inhibitor is discussed.
银和银离子具有悠久的抗菌活性和医学应用历史。然而，银对细菌的活性，以及它如何进入细胞，尚未确定。钾通道，一种膜蛋白，是一种可能的途径。添加通道抑制剂（4-氨基吡啶）来调节银的摄取可以支持这种观点。然而，抑制剂增强了银的摄取，结果却相反。我们应用冷离子阱红外激光光谱学研究了 Ag 与 Ac-Tyr-NHMe（GYG 的一部分）的复合物，后者是钾通道选择性过滤器的一部分，以考虑渗透问题。在量子化学计算的支持下，我们确定了复合物的稳定构象。这些构象强烈表明，Ag 不容易渗透钾通道。抑制剂增强作用的机制也进行了讨论。
