Erdemir Serkan, Malkondu Sait, Oguz Mehmet
Selcuk University, Science Faculty, Department of Chemistry, 42250, Konya, Turkey.
Giresun University, Faculty of Engineering, Department of Environmental Engineering, Giresun, 28200, Turkey.
Talanta. 2023 Jun 1;258:124454. doi: 10.1016/j.talanta.2023.124454. Epub 2023 Mar 13.
High levels of residual palladium can lead to serious negative health effects. Carbon monoxide (CO) is a significant gasotransmitter in transporting intermolecular and intramolecular signals to balance several physiological processes. Therefore, there is a need for rapid detection of CO and palladium residue. To address these issues, we have designed a novel light-up fluorescent probe for the detection of Pd and CO. It can not only detect Pd and CO selectively with a remarkable chromogenic and red fluorescent response over other metal ions allowing detection with naked eyes but also discriminate Pd and Pd/Pd species. The detection reaction is confirmed by HPLC analysis. The probe demonstrates biocompatibility and mitochondrial target ability for potential biological applications. The practical applications based on drug residue and soil analysis, and smartphone have been successfully performed. Bioimaging of the concentration change of Pd and CO in HeLa cells using the probe is successfully applied. Therefore, the present approach can provide early diagnosis of Pd and CO with low detection limit, low cytotoxicity, high selectivity, and sensitivity.
高含量的残留钯会对健康造成严重的负面影响。一氧化碳（CO）是一种重要的气体信号分子，在传递分子间和分子内信号以平衡多种生理过程中发挥作用。因此，需要快速检测CO和钯残留。为了解决这些问题，我们设计了一种新型的发光荧光探针用于检测Pd和CO。它不仅能够选择性地检测Pd和CO，与其他金属离子相比具有显著的显色和红色荧光响应，可实现肉眼检测，还能区分Pd和Pd/Pd物种。通过高效液相色谱（HPLC）分析证实了检测反应。该探针具有生物相容性和线粒体靶向能力，具有潜在的生物学应用价值。基于药物残留和土壤分析以及智能手机的实际应用已成功完成。使用该探针成功实现了对HeLa细胞中Pd和CO浓度变化的生物成像。因此，本方法能够以低检测限、低细胞毒性、高选择性和高灵敏度对Pd和CO进行早期诊断。
