基于多功能磁性普鲁士蓝纳米酶和胶体金的两种侧向流动免疫分析法用于检测猪尿液和猪肉中的38种β-激动剂。
Two kinds of lateral flow immunoassays based on multifunctional magnetic prussian blue nanoenzyme and colloidal gold for the detection of 38 β-agonists in swine urine and pork.
作者信息
Liang Jinxuan, Liu Zhiwei, Fang Yalin, Shen Xing, Xu Zhenlin, Lei Hongtao, Huang Xianhui, Li Xiangmei
机构信息
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
出版信息
Food Chem. 2023 Aug 15;417:135897. doi: 10.1016/j.foodchem.2023.135897. Epub 2023 Mar 11.
Herein, novel multifunctional magnetic prussian blue nanoenzymes (MPBNs) and colloidal gold (CG) were synthesized and used to develop two kinds of lateral flow immunoassays (LFIAs) for the detection of 38 β-agonists. Since MPBNs has a unique three-in-one function of colorimetric magnetic catalytic activities, the signal intensity and coupling ratio are 2 and 8-fold higher than that of the CG. The cut-off values of the CG-LFIA and MPBNs-LFIA for swine urine and pork are 5/5 and 0.3/0.5 μg/kg, the limits of detection are 0.19/0.29 and 0.02/0.03 μg/kg, respectively. The sensitivity of MPBNs-LFIA is 10-fold higher than that of CG-LFIA, and up to 200-fold higher than that of the reported LFIAs. The recoveries of the LFIAs are 80.0%-116.7%, with coefficients of variation of 1.4%-14.3%. Our study proved that the MPBNs have more advantages than CG, and can offer a promising signal label for ultrasensitive immunoassay techniques.
在此，合成了新型多功能磁性普鲁士蓝纳米酶（MPBNs）和胶体金（CG），并用于开发两种用于检测38种β-激动剂的侧向流动免疫分析方法（LFIAs）。由于MPBNs具有比色磁催化活性的独特三合一功能，信号强度和偶联率分别比CG高2倍和8倍。CG-LFIA和MPBNs-LFIA对猪尿液和猪肉的截断值分别为5/5和0.3/0.5μg/kg，检测限分别为0.19/0.29和0.02/0.03μg/kg。MPBNs-LFIA的灵敏度比CG-LFIA高10倍，比报道的LFIAs高200倍。LFIAs的回收率为80.0%-116.7%，变异系数为1.4%-14.3%。我们的研究证明，MPBNs比CG具有更多优势，可为超灵敏免疫分析技术提供有前景的信号标记。
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