Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, UP, India; Department of Biochemistry, J.N. Medical College, Aligarh Muslim University, Aligarh, 202002, UP, India.
Department of Biochemistry, J.N. Medical College, Aligarh Muslim University, Aligarh, 202002, UP, India.
Toxicol In Vitro. 2023 Jun;89:105583. doi: 10.1016/j.tiv.2023.105583. Epub 2023 Mar 15.
Hypochlorous acid (HOCl) is a major oxidant produced by activated neutrophils via the myeloperoxidase catalyzed reaction. The production of HOCl eliminates a wide range of pathogens. However, HOCl can also cause significant oxidative damage in cells and tissues where it is generated. The protective effect of curcumin was studied on HOCl-induced oxidative damage to human red blood cells (RBC). Isolated RBC were incubated with HOCl at 37 °C in absence or presence of different concentrations of curcumin. Hemolysates were prepared and assayed for various biochemical parameters. Treatment of RBC with HOCl alone increased hemolysis, protein carbonyls, heme degradation and chloramines as compared to untreated control cells. This was accompanied by reduction in glutathione level, total sulfhydryls and free amino groups. HOCl also lowered the activities of major antioxidant enzymes and diminished the antioxidant power of RBC. Pre-treatment of RBC with different concentrations of curcumin resulted in concentration-dependent attenuation in all these parameters while curcumin alone had no significant effect. Scanning electron microscopy showed that curcumin prevented HOCl-induced morphological changes in RBC and restored their normal biconcave shape. Thus curcumin can be used as a chemoprotective agent to mitigate HOCl-induced oxidative damage to cells. These results also explain the beneficial effects of curcumin against Helicobacter pylori induced stomach ulcers, caused by excessive production of HOCl at the site of bacterial infection.
次氯酸（HOCl）是一种主要氧化剂，由活化的中性粒细胞通过髓过氧化物酶催化反应产生。HOCl 的产生可以消除多种病原体。然而，HOCl 也会在产生它的细胞和组织中引起严重的氧化损伤。本研究探讨了姜黄素对 HOCl 诱导的人红细胞（RBC）氧化损伤的保护作用。将 RBC 与 HOCl 在 37°C 下孵育，有无不同浓度的姜黄素。制备溶血物并测定各种生化参数。与未经处理的对照细胞相比，单独用 HOCl 处理 RBC 会增加溶血、蛋白质羰基、血红素降解和氯胺。这伴随着谷胱甘肽水平、总巯基和游离氨基基团的减少。HOCl 还降低了主要抗氧化酶的活性，降低了 RBC 的抗氧化能力。用不同浓度的姜黄素预处理 RBC 会导致所有这些参数呈浓度依赖性衰减，而姜黄素本身没有显著影响。扫描电子显微镜显示，姜黄素可防止 HOCl 诱导的 RBC 形态变化，并恢复其正常双凹形。因此，姜黄素可用作化学保护剂，减轻 HOCl 诱导的细胞氧化损伤。这些结果也解释了姜黄素对幽门螺杆菌引起的胃溃疡的有益作用，这是由于在细菌感染部位 HOCl 的过度产生。
