Gültekin Mustafa, Ceran Sami, Gültekin Burcu
Department of Thoracic Surgery, Beyhekim Training and Research Hospital, Konya, Türkiye.
Department of Thoracic Surgery, Necmettin Erbakan University Meram Faculty of Medicine, Konya, Türkiye.
Turk Gogus Kalp Damar Cerrahisi Derg. 2023 Jan 30;31(1):87-94. doi: 10.5606/tgkdc.dergisi.2023.22694. eCollection 2023 Jan.
This study aims to compare methylprednisolone frequently used in the therapeutic practices of corrosive esophagus burns, sucralfate, a protective material of mucosal surfaces, and alpha lipoic acid, the most potent antioxidant in a rat model.
A total of 40 female Sprague-Dawley rats were used in this study. The rats were equally divided into control, alpha lipoic acid, methylprednisolone, and sucralfate groups (n=10). A corrosive esophagus burn was created by using 10% pH:12 sodium hydroxide. No treatment was applied to the control group, and each group was given their own treatment. The treatment was continued regularly until the eighth day, when they were sacrificed. The corrosive esophagus burn lines were removed and tissue sections were stained with hematoxylin and eosin.
The difference in ulceration in the group treated with alpha lipoic acid was significant, compared to the other groups. The most excellent complete epithelialization and complete re-epithelialization were observed in the alpha lipoic acid group. The difference between the groups was significant, with complete re-epithelialization being the lowest in the control and methylprednisolone groups (42.9% and 12.5%, respectively) and the highest in the alpha lipoic acid group (77.8%). In terms of ulceration and re-epithelialization, comparable values were found in the alpha lipoic acid group. The main difference was that the inflammation levels in the sucralfate group were lower and more favorable than the other groups in this period. The glutathione level was significantly higher in the alpha lipoic acid group and decreased the tissue hydroxyproline level.
Alpha lipoic acid reduces esophageal ulceration, severity and prevalence of inflammation, severity and prevalence of fibrosis, decreases tissue damage by increasing blood glutathione level, and also reduces stricture in corrosive esophagus burns in rats.
本研究旨在比较在大鼠模型中，腐蚀性食管烧伤治疗实践中常用的甲泼尼龙、粘膜表面保护材料硫糖铝和最有效的抗氧化剂α-硫辛酸。
本研究共使用40只雌性Sprague-Dawley大鼠。将大鼠平均分为对照组、α-硫辛酸组、甲泼尼龙组和硫糖铝组（n = 10）。通过使用10%pH值为12的氢氧化钠造成腐蚀性食管烧伤。对照组不进行治疗，每组给予各自的治疗。治疗持续进行至第8天，然后处死大鼠。取出腐蚀性食管烧伤部位，组织切片用苏木精和伊红染色。
与其他组相比，α-硫辛酸治疗组的溃疡差异显著。在α-硫辛酸组观察到最优异的完全上皮化和完全再上皮化。各组之间的差异显著，对照组和甲泼尼龙组的完全再上皮化最低（分别为42.9%和12.5%），α-硫辛酸组最高（77.8%）。在溃疡和再上皮化方面，α-硫辛酸组的值相当。主要区别在于，在此期间硫糖铝组的炎症水平低于其他组且更有利。α-硫辛酸组的谷胱甘肽水平显著更高，并降低了组织羟脯氨酸水平。
α-硫辛酸可减少大鼠腐蚀性食管烧伤中的食管溃疡、炎症的严重程度和发生率、纤维化的严重程度和发生率，通过提高血液谷胱甘肽水平减少组织损伤，还可减轻狭窄。
