Chelliah Ramachandran, Park Se Jin, Oh Sungyoon, Lee Eunseok, Daliri Eric Banan-Mwine, Elahi Fazle, Park Chae Rin, Sultan Ghazala, Madar Inamul Hasan, Oh Deog Hwan
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea.
Department of Food Science and Biotechnology, Kangwon Institute of Inclusive Technology (KIIT), Kangwon National University, Gangwon-do 24341, Republic of Korea.
MethodsX. 2023 Feb 26;10:102096. doi: 10.1016/j.mex.2023.102096. eCollection 2023.
Alternative bio-refinery technologies are required to promote the commercial utilization of plant biomass components. The fructooligosaccharide (FOS) obtained after hydrolysis of the hemicellulose fractions was mainly applied in the pharmaceutical and food industries. Agricultural bi-product is a rich constituent in dietary fibres, which have prebiotic effects on the intestinal microbiota and the host. Herein we explored the impact of FOS on microbiota modulation and the gut homeostasis effect. High fructooligosaccharide recovery was obtained using alkaline extraction techniques. The enzymatic method produced fructooligosaccharides with minor contamination from fructan and glucan components, although it had a low yield. But combining the alkaline and enzymatic process provides a higher yield ratio and purity of fructooligosaccharides. The structure of the fructooligosaccharide was confirmed, according to FTIR, C NMR, H NMR and 2D-NMR data. Our results could be applied to the development of efficient extraction of valuable products from agricultural materials using enzyme-mediated methods, which were found to be a cost-effective way to boost bio-refining value. Fructooligosaccharides with varying yields, purity, and structure can be obtained.
需要替代生物炼制技术来促进植物生物质成分的商业利用。半纤维素组分水解后得到的低聚果糖（FOS）主要应用于制药和食品工业。农业副产品富含膳食纤维，对肠道微生物群和宿主具有益生元作用。在此，我们探讨了低聚果糖对微生物群调节和肠道稳态的影响。使用碱性提取技术可实现高回收率的低聚果糖。酶法生产的低聚果糖受果聚糖和葡聚糖成分的污染较小，尽管产率较低。但将碱性和酶法工艺相结合可提供更高的低聚果糖产率和纯度。根据傅里叶变换红外光谱（FTIR）、碳核磁共振（C NMR）、氢核磁共振（H NMR）和二维核磁共振（2D-NMR）数据确定了低聚果糖的结构。我们的结果可应用于利用酶介导方法从农业原料中高效提取有价值产品的开发，这被认为是提高生物炼制价值的一种经济有效的方法。可以获得产率、纯度和结构各异的低聚果糖。
