Dept. of Biotechnology & Medical Engineering, National Institute of Technology Rourkela, 769008, India.
Food Microbiology and Bioprocess Laboratory, Dept. of Life Science, National Institute of Technology Rourkela, 769008, India.
Int J Biol Macromol. 2020 Jul 1;154:557-566. doi: 10.1016/j.ijbiomac.2020.03.086. Epub 2020 Mar 13.
Gut microbiome evidenced as the assembling mode of action facilitates the relationship of environmental factors (such as diet and lifestyle) with colorectal cancer. The cytotoxic and anticancer studies of the enzymatically extracted polysaccharides from selected Indian seaweeds (such as S. wightii, E. compressa, and A. spicifera) on Raw 264.7 macrophage and HT-29 human colon cancer cell line were investigated. E. compressa showed nitric oxide production up to a concentration of 6.99 ± 0.05 μM. The polysaccharide extract of seaweed (PES), A. spicifera (100 μg/ml) had shown the highest in-vitro cytotoxicity effect on HT-29 cells up to 52.13 ± 1.4%. Absorption, distribution, metabolism and excretion (ADME) predictions were performed for exploring the possibility of anti-cancer drug development. The formulated synbiotic candy exhibited post storage survivability of probiotic species L. plantarum NCIM 2083 up to 10 CFU/ml until three weeks and it could be an aesthetic functional food for treating colon cancer.
肠道微生物组被证明是一种作用方式，有助于将环境因素（如饮食和生活方式）与结直肠癌联系起来。对从选定的印度海藻（如 S. wightii、E. compressa 和 A. spicifera）中提取的酶解多糖对 Raw 264.7 巨噬细胞和 HT-29 人结肠癌细胞系的细胞毒性和抗癌研究进行了调查。E. compressa 显示出高达 6.99±0.05μM 的一氧化氮产生。海藻多糖提取物（PES）、A. spicifera（100μg/ml）对 HT-29 细胞的体外细胞毒性作用最高，可达 52.13±1.4%。进行了吸收、分布、代谢和排泄 (ADME) 预测，以探索开发抗癌药物的可能性。所配制的共生糖果在储存后仍能保持益生菌 L. plantarum NCIM 2083 的存活能力，直到 3 周，可达 10 CFU/ml，这使其成为治疗结肠癌的一种美观的功能性食品。
