Feng Guo-Ying, Zhang Nai-Li, Liu Xiao-Wei, Tong Ling-Xi, Zhang Chun-Lei, Zhou Shuai, Zhang Lu-Ping, Huang Fei
Institute of Neurobiology, Binzhou Medical University, Laishan, Shandong Province, China.
Institute of Neurobiology, Binzhou Medical University, Laishan; School of Health and Life Sciences, University of Health and Rehabilitation Sciences, Qingdao, Shandong Province, China.
Neural Regen Res. 2023 Sep;18(9):1956-1960. doi: 10.4103/1673-5374.367974.
Studies have shown that serum response factor is beneficial for axonal regeneration of peripheral nerves. However, its role after central nervous system injury remains unclear. In this study, we established a rat model of T9-T10 spinal cord transection injury. We found that the expression of serum response factor in injured spinal cord gray matter neurons gradually increased with time, reached its peak on the 7 day, and then gradually decreased. To investigate the role of serum response factor, we used lentivirus vectors to overexpress and silence serum response factor in spinal cord tissue. We found that overexpression of serum response factor promoted motor function recovery in rats with spinal cord injury. Qualitative observation of biotinylated dextran amine anterograde tracing showed that overexpression of serum response factor increased nerve fibers in the injured spinal cord. Additionally, transmission electron microscopy showed that axon and myelin sheath morphology was restored. Silencing serum response factor had the opposite effects of overexpression. These findings suggest that serum response factor plays a role in the recovery of motor function after spinal cord injury. The underlying mechanism may be related to the regulation of axonal regeneration.
研究表明，血清反应因子有利于周围神经的轴突再生。然而，其在中枢神经系统损伤后的作用仍不清楚。在本研究中，我们建立了T9 - T10脊髓横断损伤的大鼠模型。我们发现，损伤脊髓灰质神经元中血清反应因子的表达随时间逐渐增加，在第7天达到峰值，然后逐渐下降。为了研究血清反应因子的作用，我们使用慢病毒载体在脊髓组织中过表达和沉默血清反应因子。我们发现，血清反应因子的过表达促进了脊髓损伤大鼠的运动功能恢复。生物素化葡聚糖胺顺行示踪的定性观察表明，血清反应因子的过表达增加了损伤脊髓中的神经纤维。此外，透射电子显微镜显示轴突和髓鞘形态得以恢复。沉默血清反应因子产生了与过表达相反的效果。这些发现表明，血清反应因子在脊髓损伤后运动功能恢复中起作用。其潜在机制可能与轴突再生的调节有关。
