Wang Haitao, Jurasinski Gerald, Täumer Jana, Kuß Andreas W, Groß Verena, Köhn Daniel, Günther Anke, Urich Tim
Institute of Microbiology, University of Greifswald, 17489 Greifswald, Germany.
Landscape Ecology, University of Rostock, 18059 Rostock, Germany.
Environ Sci Technol. 2023 Mar 28;57(12):5089-5101. doi: 10.1021/acs.est.2c07461. Epub 2023 Mar 16.
Rewetted peatlands are reestablished hot spots for CH emissions, which are subject to increased drought events in the course of climate change. However, the dynamics of soil methane-cycling microbiomes in rewetted peatlands during summer drought are still poorly characterized. Using a quantitative metatranscriptomic approach, we investigated the changes in the transcript abundances of methanogen and methanotroph rRNA, as well as and mRNA before, during, and after the 2018 summer drought in a coastal and a percolation fen in northern Germany. Drought changed the community structure of methane-cycling microbiomes and decreased the CH fluxes as well as the rRNA and mRNA transcript abundances of methanogens and methanotrophs, but they showed no recovery or increase after the drought ended. The rRNA transcript abundance of methanogens was not correlated with CH fluxes in both fens. In the percolation fen, however, the transcript abundance showed a positive and significant correlation with CH fluxes. Importantly, when integrating abundance, a stronger correlation was observed between CH fluxes and /, suggesting that relationships between methanogens and methanotrophs are the key determinant of CH turnover. Our study provides a comprehensive understanding of the methane-cycling microbiome feedbacks to drought events in rewetted peatlands.
重新湿润的泥炭地是甲烷排放的重新形成的热点区域，在气候变化过程中易遭受更多干旱事件。然而，夏季干旱期间重新湿润的泥炭地中土壤甲烷循环微生物群落的动态变化仍缺乏充分描述。我们采用定量宏转录组学方法，研究了德国北部一个沿海泥炭地和一个渗流沼泽在2018年夏季干旱之前、期间和之后产甲烷菌和甲烷氧化菌rRNA以及 和 mRNA转录丰度的变化。干旱改变了甲烷循环微生物群落的结构，降低了甲烷通量以及产甲烷菌和甲烷氧化菌的rRNA和mRNA转录丰度，但干旱结束后它们没有恢复或增加。两个泥炭地中产甲烷菌的rRNA转录丰度与甲烷通量均无相关性。然而，在渗流沼泽中， 转录丰度与甲烷通量呈正相关且显著相关。重要的是，当整合 丰度时，在甲烷通量与 / 之间观察到更强的相关性，这表明产甲烷菌与甲烷氧化菌之间的关系是甲烷周转的关键决定因素。我们的研究全面了解了重新湿润的泥炭地中甲烷循环微生物群落对干旱事件的反馈。
