Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.
Chronobiol Int. 2023 May;40(5):569-580. doi: 10.1080/07420528.2023.2189481. Epub 2023 Mar 17.
Feeding rhythms affect a range of physiological functions in crustaceans. To investigate their effect on the physiological functions of , herein, we analyzed the influence of different feeding times on the hepatopancreas transcriptome via high-throughput sequencing. We harvested the hepatopancreas of crabs at 12:00 on day 11 of the experiment. We weighted the crabs before and after the experiment and found that those in the 06:00 group had the highest weight gain rate. In addition, 512 differentially expressed genes (DEGs) were grouped into nine distinct clusters. Functional enrichment analysis of DEGs showed that metabolic and immune processes were affected by the feeding time. Furthermore, we mapped the DEGs involved in retinol metabolism and the lysosome pathway. To our knowledge, this is the first comparative transcriptomic analysis of the hepatopancreas of based on different feeding times, which provides multi-level information to reveal the mechanism underlying the regulation of feeding rhythms in .
摄食节律会影响甲壳动物的一系列生理功能。为了研究其对 生理功能的影响，本研究通过高通量测序分析了不同摄食时间对蟹肝胰腺转录组的影响。实验第 11 天 12:00 时采集蟹肝胰腺，实验前后称蟹体重，发现 06:00 组的增重率最高。此外，共鉴定出 512 个差异表达基因（DEGs），分为 9 个不同的簇。DEGs 的功能富集分析表明，代谢和免疫过程受到摄食时间的影响。此外，我们还对涉及视黄醇代谢和溶酶体途径的 DEGs 进行了映射。据我们所知，这是首次基于不同摄食时间对 肝胰腺进行的比较转录组分析，为揭示 摄食节律调控机制提供了多层次信息。
