Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
J Vet Med Sci. 2023 May 3;85(5):532-535. doi: 10.1292/jvms.22-0544. Epub 2023 Mar 14.
The poultry red mite (PRM; Dermanyssus gallinae) is a hematophagous ectoparasite that mainly infests chickens, and its infestation causes significant economic losses to the poultry industry. In this study, we examined the use of RNAscope-based in situ hybridization (ISH) to characterize gene expression in PRM. We analyzed the mRNA expression of Dermanyssus gallinae cathepsin D-1 (Dg-CatD-1) and Dermanyssus gallinae cystatin (Dg-Cys). RNAscope ISH analysis revealed that mRNA expression of Dg-CatD-1 was observed in the digestive tract, and Dg-Cystatin mRNA was expressed in the ovaries in addition to the digestive tract. RNAscope ISH could be applicable for the analysis of gene expression in each tissue of PRM and is an effective method to investigate the characteristics of target genes.
家禽红螨（PRM；Dermanyssus gallinae）是一种吸血的外寄生虫，主要侵袭鸡，其感染给家禽业造成了重大的经济损失。在这项研究中，我们研究了基于 RNAscope 的原位杂交（ISH）在 PRM 基因表达特征分析中的应用。我们分析了 Dermanyssus gallinae 组织蛋白酶 D-1（Dg-CatD-1）和 Dermanyssus gallinae 半胱氨酸蛋白酶抑制剂（Dg-Cys）的 mRNA 表达。RNAscope ISH 分析显示，Dg-CatD-1 的 mRNA 表达存在于消化道中，而 Dg-Cystatin mRNA 除了在消化道中表达外，还在卵巢中表达。RNAscope ISH 可适用于 PRM 每个组织中基因表达的分析，是研究靶基因特征的有效方法。
