Institute of Emergency, Medicine Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic.
Centre for Advanced Innovation Technologies, VŠB - Technical University of Ostrava, Ostrava, Czech Republic.
Histol Histopathol. 2023 Oct;38(10):1119-1127. doi: 10.14670/HH-18-606. Epub 2023 Mar 13.
Fractured bones can regenerate and restore their biological and mechanical properties to the state prior to the damage. In some cases, however, the treatment of fractures requires the use of supportive implants. For bone healing, three processes are essential: the inflammatory phase, the repair phase and the remodelling phase. A proper course of the first - inflammatory - stage is important to ensure a successful fracture healing process. In our study, we evaluated tissue samples immunohistochemically from the area surrounding the fractures of upper and lower limbs (bone tissue, soft tissue, and the implant-adhering tissue) for markers: CD11b, CD15, CD34, CD44, CD68, Cathepsin K, and TRAcP that are linked to the aforementioned phases. In soft tissue, higher expressions of CD68, CD34, CD15 and CD11b markers were observed than in other locations. TRAcP and Cathepsin K markers were more expressed in the bone tissue, while pigmentation, necrosis and calcification were more observed in the implant-adhering tissue. Since even the implant materials commonly perceived as inert elicit the observed inflammatory responses, new surface treatments and materials need to be developed.
骨折可以再生，并恢复其生物和机械性能，使其恢复到损伤前的状态。然而，在某些情况下，骨折的治疗需要使用支持性植入物。对于骨愈合，有三个过程是必不可少的：炎症期、修复期和重塑期。适当的第一阶段——炎症期——对于确保骨折愈合过程的成功是很重要的。在我们的研究中，我们对上肢和下肢骨折周围的组织样本（骨组织、软组织和与植入物附着的组织）进行了免疫组织化学评估，以寻找与上述各期相关的标志物：CD11b、CD15、CD34、CD44、CD68、组织蛋白酶 K 和 TRACP。在软组织中，观察到 CD68、CD34、CD15 和 CD11b 标志物的表达高于其他部位。TRACP 和组织蛋白酶 K 标志物在骨组织中表达较多，而在与植入物附着的组织中则更多地观察到色素沉着、坏死和钙化。由于即使是被普遍认为惰性的植入材料也会引起观察到的炎症反应，因此需要开发新的表面处理和材料。
